Critical thinking courses Vermont Academy
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CRITICAL THINKING - Fundamentals: Introduction to Critical Thinking HD
Geoff Pynn (Northern Illinois University) gets you started on the critical thinking journey. He tells you what critical thinking is, what ...
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essay short Short Essay Samples. Brought to you by EssayEdge.com -- The world's premier Application Essay editing service -- The New York Times Learning Network. Please select from the thinking Vermont following short essay sets: Note: The below essays were not edited by EssayEdge Editors. They appear as they were initially reviewed by write an essay School admissions officers.
SAMPLE SHORT ESSAY SET 1: Georgetown, Saudi International Relations. For many years, I have been interested in studying international relations. My interest in thinking Academy, pursuing this field stems from several factors which have affected me. First, I have been exposed to international affairs throughout my life. Write An Essay School! With my father and two of my brothers in the Saudi Foreign Service, I have grown up under the shadow of inter-national affairs. Second, I am fascinated by thinking Vermont history, economics, and diplomacy. I believe, through the online School study of international relations, I can effectively satisfy my curiosity in thinking Vermont, these fields.
A third factor which has affected my interest in international relations is patriotism. Through the Foreign Service, I would not only have the opportunity to serve my country, but also have the chance to how to write an essay for kids help bridge gaps between my country and others. Finally, as a Saudi living abroad, I have been bridging cultures throughout my life. This experience has taught me to critical thinking courses Academy look for differences to what University compromise and thinking courses Vermont Academy, similarities to synthesize in order to balance different cultures. How To Essay Deakin University! In short, I believe that my experiences in life, combined with a rigorous academic education, will enable me to pursue a successful career in the Saudi Foreign Service. Georgetown, Favorite Class.
At St. Albans, especially in our later years, we are given the freedom to choose from a vast array of classes. Thinking Courses Vermont! Using this freedom, I have selected classes which have personal significance to me, regardless of difficulty or appearance on my transcript. Write Ross! However, from thinking courses Academy, these classes, one holds an extraordinary amount of value to me. This course is A.P. Omnibus History, a combination of American and European history.
There are several reasons for my great interest in a essay SDA Bocconi School of Management, this class. First, I am fascinated by the cyclical nature of the past. I see these recurring political, economic, and social trends as a means of looking forward into the future, while allowing us to avoid the mistakes of the past. Second, history teaches many lessons about the nature of critical courses, human behavior, both past and present, providing insight into the actions, desires, and aspirations of those around me. Jiaotong-Liverpool! Finally, it lays a solid foundation for several disciplines, including political science, economics, and international relations, three fields of great interest to me. Georgetown, Visual Arts. Another major interest of mine, which I have not had the opportunity to express elsewhere on my application, is the visual arts. Throughout high school, I have used a variety of thinking courses Vermont Academy, media to express myself. I began with black and white photography, focusing on the presence of lines and write of Brighton, balance in nature.
For my work in this medium, I received an award at the St. Albans School Art Show. Thinking Courses Vermont Academy! From photography, I moved on to glass etching. Using a sandblaster to etch the glass, I again concentrated on lines and balance in help School of Management, my works. Moreover, by arranging several glass panes into a sculpture, I moved my study into three dimensions, winning another Art Show award. Currently, I am working on critical Academy canvas, using oil and acrylic in a Mondrian style, which is based on lines and balance. Eventually, I hope to explore the effects of combining these and write my essay online Ross, other media, creating my own style of artistic expression.
In the thinking courses Vermont past four years of write Deakin (Navitas), my life, no activity has affected me more than wrestling. Four years of varsity wrestling and the honor of critical Academy, being a team captain has instilled many qualities in how to write Deakin University, me. First, through years of hard work and thinking courses Vermont Academy, continuous dieting, wrestling has given me discipline. This discipline has spread to other parts of my personality, including my moral character, work ethic, and perserverence. Another quality wrestling has given me is leadership.
As a team captain, I have learned to lead by example, both on and off the how to University (Navitas) mat. Above all, though, wrestling has given me a love of life. Through this sport, I have experienced pain, sacrifice, adversity, and success. Exposure to these feelings-which are, in my opinion, the essence of critical thinking courses Vermont Academy, being-has allowed me to truly appreciate life. I hope to continue wrestling at Georgetown. What immediately strikes the reader about this set-before even reading it-is the balance between the essays. Each answer contains only one paragraph, each of approximately equal length. The solid structure of each essay and the focus of each reflects this outward balance.
Each one focuses on a completely different area of its writer’s life, another striking detail. Write The University! The first focuses on his career goals, the second on his interest in history, the third on his interest in the visual arts, and the fourth on wrestling. This is thinking courses Academy a perfect example of the how to write an essay for kids jigsaw puzzle approach. When put together, you have a well-rounded individual with passion, depth, and critical Vermont Academy, involvement in many different areas. SAMPLE SHORT ESSAY SET 2: Throughout my life, I have tried to be a well-balanced person. Growing up in the South, I had a hard time fighting the stereotypical image of a Chinese person. How To Write Essay Plan University! I was expected to be a math and science genius and critical thinking Academy, nothing more. As it turned out, I defied my detractors by excelling in English and history along with math and science.
And over write an essay for kids The University the years, I have continued to maintain my academic standards. Nevertheless, I have also made sure that I am more than an academic person. I am an active one as well. In middle school, the most popular game during lunch was a basketball game called Salt and Pepper (white vs. Critical Courses! black). How To Deakin (Navitas)! The first day of school, I stepped onto the basketball courts and was greeted by cries of consternation, “Who is thinking courses he? Is he salt or pepper?” But after the online Ross School game, I had made a name for myself.
From then onward, I would be known as Spice, and the game we played became Salt, Pepper, and Spice. When I moved to critical courses Vermont Academy California, things were no different. I continued to in english Windermere School play an active part both academically and socially. My involvement with Cross-country, Speech and Debate, Ultimate Frisbee and thinking courses Vermont, numerous clubs guaranteed that I would not be only known as an Honors student. Like myself, Duke is much more than an academic institution; it is a living institution.
I feel that I will be given the opportunity to excel both academically and socially. Duke is a university known for its rich history and an essay School, strong academic program. And, at the same time, it is thinking courses Vermont Academy also known for its innovation and writing School of Management, progressiveness. These are qualities which draw me to the college. In addition, Duke and critical, I have a lot in how to for kids The University, common. The two most important extracurricular activities I have are a major part of Duke University.
Duke’s Speech team is known for its strong Extemp squad. I remember the critical Vermont time when my speech coach asked me what schools I was applying to. When I had listed my top five choices, he frowned at how to plan, me and said, “Out of all those schools, I will only respect you if you either join us at Berkeley or go to Duke and extemp.” I hope I will be given the opportunity to contribute my part in the Duke Speech team. Equally important, the Duke University has a well-known Ultimate Frisbee team. Critical Thinking Vermont Academy! I look forward expectantly to becoming a part of the team. How To University! Strange as it seems, Ultimate Frisbee is one of my top criteria for critical Vermont choosing my future college. It delights me that Duke places such great emphasis on the two extracurricular activities that mean most to me. My first year at Duke should be a great one. Help Writing! Majoring in economics at Duke should allow me to both pursue my major studies and allow me time for personal interests in Chinese and the Humanities.
Moreover, in my spare time, I plan to join the Speech team and critical, the Ultimate Frisbee team. Hopefully, with my previous experience, I will have an early start in Windermere, both Speech and Ultimate. Yet, I will never forget why I’m in college in the first place. As long as I give organic chemistry a wide berth, I should be able to continue my level of academic excellence. Critical Thinking Courses Vermont! Overall, my first year at Duke promises to be exciting, if a bit hectic. I find Hermann Hesse’s book, Narcissus and Goldmund, intellectually exciting. My Essay Online Ross School! After reading the book last year, I remember putting it down and sighing contentedly. Vermont Academy! I had, after a sleepless night, finally finished.
What I reveled in was not the fact that I could sleep, but that I had come away with an inexplicable something. It was not an understanding which could be pinpointed and explained. For Kids Of Brighton! Rather, it was a sense I felt in the depths of my soul. And yet, what delighted me more was that I knew that I had only begun to understand the thinking courses book; that there remained countless messages which I could only write an essay for kids The University of Brighton sense but not grasp. Here, finally, I had a book which could be re-read. And every time I finished, I would come away with a new understanding of critical thinking Vermont Academy, something I could not put into write an essay in english School, words. Unlike the normal academic, I do not want to Vermont Academy find the final answer for an essay The University of Brighton everything.
Throughout my life, I have always felt a sense of critical thinking courses Vermont, loss after succeeding in help writing SDA Bocconi School, a long search. For me, it is not the critical courses Vermont Academy ends I seek, but the words writing Nuova means themselves. I am perfectly content to never find the final answer as long as I will always be able to thinking Vermont find a better one. Duke, Chinese Culture/Economics. Born in Taiwan, I came to the United States when I was five. Armed with only two words (“hello” and “popcorn”), I braved the for kids uncertainties of critical thinking Vermont Academy, a complex, new environment. Twelve years later, my vocabulary is considerably larger and I have adapted well to my surroundings. At the write School same time, I have neither forgotten my native culture nor its language. My ties with my native Chinese culture remain as strong as ever.
I visit my relatives in Taiwan regularly almost every summer and have traveled throughout China. And to critical thinking everyone’s continuing surprise, I have yet to forget how to write for kids of Brighton speak Mandarin. Nevertheless, twelve years in thinking courses Vermont Academy, America has made its impressions upon me as well. I am as “American” as anyone my age. The songs I listen to, the sports I play, and write in english, the way I speak are all a reflection of that. In short, I am a combination of both East and West. Nevertheless, I sometimes wonder whether speaking Chinese at critical Vermont, home and what Jiaotong-Liverpool University, visits in the summer are enough to maintain my ties with my native culture. Often, when I see my parents reading old Chinese literature or poetry, I feel that I am only in touch with half of what I am. This sense of loss has led me to seek out my old roots. I turn to the East to rediscover what I have lost.
Yet, I cannot resign myself to merely studying my own culture and language. I want to be able to critical thinking Vermont Academy apply my knowledge as well. To me, pursuing a career in business is a very pragmatic solution to writing NABA, Accademia Milano my future welfare. My father is thinking Vermont a businessman in Taiwan and I have had numerous opportunities to watch him work. Through him, I have discovered my own interests in the business field. I find the way business operates in the East to be very exciting. At the same time, my father has soothed my sense of morality by showing me that it is possible to be an honest businessman in Asia. Before I learned about Duke, I had made up my mind to School study economics and to ultimately pursue a career in international business. Thinking Courses Academy! I had come to see this path as the best combination for fulfilling both my aspirations towards knowledge and Xi’an Jiaotong-Liverpool, my pragmatic goals of a future livelihood. China, my planned area of focus, is an expanding market with a dearth of critical courses, skilled business professionals. But I had misgivings because I wanted a school with a strong focus on the humanities as well.
Thus, I find Duke University exciting and perfect for me. It gives me a strong economics curriculum, but still allows me to pursue my interests in the humanities. With economics at Duke University, I will have access to what University a wide array of studies both within and beyond my chosen major. Courses! I will have an edge in the business world by virtue of writing a essay SDA Bocconi, Duke. After attending Duke (if I am accepted, of course), I will have a clear path before me. My studies at Duke should virtually guarantee me for any graduate business school. And, after my graduate studies, I will be able to thinking Vermont realize my dreams.
Perhaps, I will be able to serve as a bridge between East and West. These three well-written essays create a strong set. How To Essay Deakin (Navitas)! The first and critical Vermont, the last would have been impressive on their own. Reading them all together magnifies their impact considerably. For Essay Nuova Di Belle Arti Milano! This student does an especially good job of targeting the school. This student focuses his first essay on his extracurriculars and relates them to why Duke would be perfect for critical courses Vermont Academy him. He focuses the third on his Chinese background and how it relates to his career goals and academic interests. Then he also relates these interests to why Duke matches him perfectly. His favorite book provided the focus of the second essay. What makes this second essay better than others like it is that the vocabulary words writing Nuova Accademia applicant manages to courses Academy put himself into the question. He does not just talk about the book, he uses it to talk about himself and stress the inquisitive nature of words NABA, Nuova, his personality-always a plus.
SAMPLE SHORT ESSAY SET 3: Participating in critical thinking, my high school’s debate program has been my most meaningful activity these past four years. I have learned how to speak in front of a crowd without becoming nervous, how to think on my feet, and how to argue the merits of any side of an issue. Being on what is articles the debate team also allows me to critical educate myself on current topics of global importance such as the homeless problem, health care, and pollution. Throughout the three years I have dedicated to the activity, (high school) has always maintained a successful squad and I am quite proud to how to know that I have earned many of the thinking courses Vermont trophies and awards that have helped make the program so successful and (high school) well known on the debate circuit. Because of the activity, I have learned that from education to communication, from argument to enlightenment, debate is necessary for two or more humans to transcend mere exchange of thought and achieve synergy instead. I now view success in debate as far more than a trophy; I now see it as evidence that I can successfully communicate my beliefs to others and how to essay Deakin, have them logically accept them as their own, thus priming me for any future challenges involving human interaction. Dartmouth, Honors and Awards.
My most important honors since tenth grade have been winning the Brown University Book Award for my skills in English, being named as a National Merit Semifinalist (Finalist status pending), winning the Journalism Education Association National Write-off Award of Excellence in the Editorial division at a national conference, being selected as a Semifinalist in the NCTE Writing Contest for my work in prose, being named as an Illinois State Scholar for thinking Academy my academic achievement in vocabulary writing NABA, Nuova Accademia Arti, high school and my high A.C.T. scores, being selected to the Spanish Honor Society for my consistent success with the language in the classroom, being selected as the thinking Vermont Academy Student of the words for essay writing NABA, Nuova Arti Milano Month in thinking Vermont, the Foreign Language/Social Sciences division two years in a row for vocabulary words for essay NABA, Nuova Accademia Arti my success in those classes, and in a culminating event, being featured in Who’s Who Among American High School Students for my overall scholastic success. Dartmouth, Summer at Dartmouth. Most of my past summer was spent away from home. In that brief month in which I remained in (town name) I worked at (job) in order to earn the money I was going to spend on my trips. My first excursion was to the east coast where I visited several schools and critical courses, took in the atmosphere of an area to words writing Nuova di Belle which my midwestern self was somewhat unaccustomed. One school I was considering that I did not visit was Dartmouth. After all, I spent a month there later in the summer.
As a participant of the Dartmouth Debate Institute I spent a lot of time in Feldberg, Dana, and Baker libraries; resided in the well-known Choates; attended sessions in Silsby; and thinking Vermont, dined in the Full-Fare section of Thayer. There was also time for recreational activities such as rope swinging, volleyball, frisbee, sleep (every little bit was cherished), and beautiful hikes up to Dana. I did manage to sit down and work in such a clean, open environment, however. The instructors made sure of that. The four-week institute honed my skills in speaking, researching, structuring arguments, and thinking. As a result, my partner and I were able to help SDA Bocconi School break into critical thinking courses Academy, the elimination rounds at the institute-ending tournament which included the top debaters in help writing a essay SDA Bocconi, the nation. Aside from the debate skills I learned, I found the institute very favorable because of the exchange of ideas taking place between the students and staff. What I learned from critical courses Vermont, those exchanges enlightened me not only as a debater but also as a person. Although I enjoy all of my subjects, I regard classes I have taken in how to plan University, the social sciences to critical courses Academy be the most meaningful. Whereas some classes use formulas to describe natural occurrences, the writing di Belle Arti Milano social sciences show that not everything is explicable in courses, such a clear-cut manner. The social sciences describe people; they describe the people who make up the formulas and how and why that was done.
The social sciences also explain the past so as a society, people can avoid past catastrophes and build upon past successes. Not only do they describe how we act as we do, but why we act as we do. I am not a student who always likes to follow someone else’s rules. While most subjects allow for free thought, the social sciences encourage innovative thinking. Those classes expect students to explain why something happened based on write my essay online School certain conditions. I didn’t learn that the Iron Curtain was an economic measure in critical courses Vermont, any math class. As a student my ultimate goal is to understand things.
I feel the best way to write in english understand is not by reciting another’s thought, but by formulating my own and debating it with people who disagree with me. I believe that exchange of thought is vital in every curriculum, but the social sciences do the thinking courses Academy most to promote that exchange. I highly doubt that anyone will be debating Einstein’s ideas in the near future-and be right. This essayist dedicates the first essay to his involvement in vocabulary words writing NABA, Nuova Accademia di Belle Milano, debating. He manages to communicate quite a lot in a short amount of space (what he has learned, what he has achieved, and what debating means to him) without ever losing his focus.
The second essay is an critical thinking courses Vermont, example of an answer to a list question (“List your honors and awards”). Help Writing A Essay SDA Bocconi! The third gets more personal by describing the critical courses Vermont summer he spent at Dartmouth. The strength of this essay is that he sells himself on words writing di Belle his knowledge and familiarity of the thinking school. The weakness of this essay is that he tries to do too much and loses his focus after the second paragraph. The conclusion does not seem to fit with the points he has made in the essay-the last line particularly seems to come from nowhere. SAMPLE SHORT ESSAY SET 4: Harvard, Favorite Books.
The novel Black Like Me was the most stimulating book I have recently read. I was taken aback by the cruelty the narrator experienced when he was black compared to the hospitality he found as a white man. Possessing the help writing of Management same occupation, clothing, wealth, speech, and identity did not matter when his skin was another color. Given that this was a non-fictional piece, my reaction was even stronger. The book made me favor equality of critical thinking, opportunity for all in every endeavor so others’ opinions of them are based on (Navitas) performance, not preconceptions. Harvard, Favorite Teacher.
I selected Mr. Thinking Vermont Academy! (name) because he taught me more than U.S. History; he taught me how to think independently. An Essay For Kids The University Of Brighton! This wasn’t done only to prepare me for critical courses the free-response section of the A.P. A Essay Of Management! test, either. I know he did it to make his students responsible citizens and responsible adults. From the outset, he wanted to make sure that we knew how we stood in our political philosophy: strict constructionists or loose constructionists. He wanted to make sure that we didn’t gravitate towards empty categories like liberal or conservative, but rather focused on issues separately whenever we needed to thinking Vermont Academy take a stand on write my essay School them. Imagine my surprise when I, the son of two very conservative parents who constantly bombarded me with their rhetoric, discovered that I had strong liberal tendencies on some issues. Aside from political affiliations, Mr. (name) taught us how to make sense out of history by trying to understand the personal motives that went in to any chain of historical occurrences. In his class, I came to the realization that history isn’t only a series of names and dates printed in a textbook, but a more complex subject that requires deep thought and Vermont, analysis for write plan full comprehension. Because of Mr. (name), history is thinking courses Academy now my favorite subject.
He has also been a motivating force outside of the classroom. He always had faith in my ability and constantly encouraged me to do my best. I believe he respected my abilities and wanted to see them developed further. In fact, had it not been for School his faith in me, I would have never applied to Harvard, the school I plan to attend in critical courses Vermont Academy, the fall. Harvard, Unnoticed Accomplishment. It’s not that I’m a weak guy, just that I had been somewhat self-conscious about my strength early on in my high school career. My gym class didn’t help too much, either. Thanks to a demeaning test of strength appropriately dubbed the “Grip Test,” once each quarter I was provided the opportunity to squeeze a gadget, get a score, and have my teacher announce it out loud, no matter how high or (as in my case) how low it was. No matter how hard I tried, the cruel and callous scale never registered above 40. Almost every other male in the class could boast of a high-40’s or mid-50’s score.
I hated that test with a passion. Until recently. When this semester rolled around and I had the gripper placed in my palm, I was prepared for the same old same old. I had been improving slightly from quarter to words for essay writing NABA, di Belle Arti Milano quarter, but nothing impressive ever happened. I drew in a deep breath, squeezed, looked at the scale, and almost fainted. Sixty-six! In a way only a teenager can appreciate, for an accomplishment only a teenager would find meaningful, I thought I was in heaven.
My success was even sweeter as I watched jocks pale in critical Academy, comparison when they took the test. Sure, to some people my academic accomplishments seem fairly impressive, and I would agree. Yet the grip test situation was much more personal and represented success in an area I normally don’t pay attention to. Plus I learned two things. One: I can pride myself on the smallest triviality. Writing! Two: I’m glad we don’t measure strength in critical thinking Academy, our gym classes with the bench press. Harvard, Leadership through Dedication. To me, leadership does not necessarily mean accumulating as many titles as possible in school activities; I feel one leads through his dedication, actions, and contributions.
I have always tried to how to write The University lead in almost everything I set out to do. I feel I have been successful at that. Superficially, I have earned such titles as president of the National Honor Society chapter at my school, Editor-in-Chief, columnist, Investigative Editor, and Editorial Editor of the school newspaper, senior varsity leader in debate, and critical thinking Academy, a Class Representative for Student Council. How To Write An Essay For Kids! However, those titles don’t begin to tell the story of my abilities as a leader. They don’t reveal how I volunteered to help out at a handicapped lock-in at an unfamiliar youth center when no one else wanted to, they don’t reveal how I always sought to be on time for critical courses Vermont Academy work and to avoid boondoggling, they don’t reveal how I aided younger debaters with their argumentation so they can have the same success I was lucky enough to enjoy, they don’t reveal how I became a role model for the JETS squad by studying my material often, eventually becoming the write my essay online School most medaled member on the team, and they don’t reveal all the thinking effort I put into learning my lines and acquiring a good stage presence for Images, my first stage production ever, so I wouldn’t single-handedly jeopardize the whole show with my lack of experience. All those actions stress the quality I feel is most important in a leader, dedication. With dedication comes hard work and the ability to seek out solutions when problems get in the way, whether they are with a news page layout or in a student’s diction. Because of this dedication, taking charge is second nature for me. People are always willing to follow one with a clear sense of direction. Harvard, Close-knit family. I don’t view my important characteristics as different from those my family has imparted on what Jiaotong-Liverpool University me throughout the years.
The pride, care, dedication, effort, and hard-working attitude that I view as critical to any success I may achieve have all descended upon critical thinking Vermont Academy me courtesy of my close-knit, Italian family. Born the child of two immigrants who came here with nothing, only one possessing a college degree, the importance of a good work ethic was stressed by my parents from day one. Through their actions in their jobs and through the verbal lessons on life I began to get from the words for essay writing NABA, Nuova di Belle moment I could communicate, they set an example for me to follow, one of being proud of what I do, no matter what it was, and above all, to care about everything I do as if everything had a big impact. Thinking Courses Vermont Academy! This meant that everything had to be done right and be done well. Undoubtedly, following their own advice carried my parents from their status as blue-collar immigrants who labored as a factory workers to white-collar citizens, one of whom owns his own business while the words for essay NABA, Nuova di Belle Arti Milano other works as a bank officer. Those ascensions from nothing only thinking Academy served as other examples for me to follow, examples that delineated the ability for a person to improve through effort. Another quotation from my father propelled me from the time I started school to my essay online Ross today: “No matter what you do, you have to be the best.” This set up the inner drive that motivates all my actions. It was what forced me to critical courses try hard in school although I didn’t know English well enough to always understand the write essay teacher. It’s the reason why I have developed my skills. Thinking Courses Vermont! It accounts for my dedication to for kids of Brighton all activities, and to the hard work I put into all of them as I strive to lead both in class and out. Essentially, my parentage was the first quality that distinguished me as a leader.
Despite all the talk of thinking courses, being a leader, I have never lost sight of the importance of my family. I know I owe my family everything, and as a result, I’ll always be close with it. I pursue a variety of an essay in english Windermere School, activities for fun and relaxation. Critical Thinking Courses! I enjoy reading books and magazines (my tastes range from Time to Gentlemen’s Quarterly) on a regular basis, imitating Beavis and Butt-head, and most of write Windermere, all, spending time with my friends. Although I am fan of playing pick-up games of basketball, football, and roller hockey, the phrase “doing nothing with my time” doesn’t bother me since I can have a good time just hanging around. I think people, not places, make for a good time. Harvard, Social Concerns. My major social concerns all revolve around the future.
In other words, I’m concerned about what prevents people from rising above their disadvantages. Critical Courses! Specifically, I am most concerned with the handicapped, education, and crime. I feel society’s response to handicaps is what is articles Xi’an Jiaotong-Liverpool University what really hampers the potential of the disabled. Critical Courses Vermont! It is important for help writing a essay SDA Bocconi of Management the disabled to get a better sense of worth and to be able to adapt to, and survive in, today’s world. Through National Honor Society (NHS), I have done just that. Courses Academy! I have helped out at a lock-in that was designed to foster interaction among the children of the organization, as well as at Special Olympics, where the children participate in sports on a competitive basis so their talents and abilities can be recognized. Whenever the disabled can be successful at my essay online School, an activity, the barrier between them and the rest of society is drastically reduced. Education is key to other problems such as gangs, drugs, and crime because it can prevent and eliminate them. I try to Vermont Academy get students in our school to maximize their opportunities by using the educational resources available. By setting up a tutoring program through NHS, I have matched up needy students with other students who can assist them with their problems in classes.
More directly, I help students out writing a essay SDA Bocconi, with English and show them how to thinking courses use the Writing Center Lab, an indispensable resource for writing a essay SDA Bocconi English students at any level. The more educated a person is, I believe, the more able he is to be successful in Vermont, the future. I have dealt with criminal problems in my school by discussing solutions to gangs and other crime in the Student Advisory Committee. We have drafted several proposals to help reduce those problems in our school. Educating people about such social concerns is also very crucial because they won’t fix what they don’t think is broken. That is one objective of our newspaper, in which we have written various editorials and news stories to educate the student body on social topics. Through debate, I myself have become knowledgeable on how to an essay for kids The University of Brighton such topics as the homeless, poverty, health care, and the environment. That way I can practice what I preach. Harvard is notorious for thinking courses Vermont Academy its long list of essay questions, as you can see from the what is articles University seven essays this applicant had to critical courses Vermont write. The first essay is a standard favorite book essay.
His second, about his favorite teacher, goes into more depth and reveals more about the candidate, that he enjoys learning, admires independent thought, and plans to study history. The third essay in this set stands out from the rest. Had the panel who were grading the compositions understood the context of this essay in light of the six others in the set, they probably would have given it more credit. Its strength lies in its funny, lighthearted approach-it shows a completely different aspect of the candidate’s personality. Without it, he would have appeared deadpan serious and probably a bit dull.
However, showing the my essay Ross wittier side of himself strengthens the set considerably. It is a good example of allowing yourself to take a risk in thinking Vermont, one essay, as long as more serious approaches in the others balance it.
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Examining the Rna Interference Mechanism Essay Sample. RNA interference, or RNAi, is a biological process in which RNA molecules reduce the gene expression of an organism. Thinking Courses Vermont! This is done typically by causing the destruction of specific mRNA molecules. RNAs are direct products of genes, these small RNAs can bind to other mRNA molecules to either increase or decrease their activity like in the example of preventing an mRNA from producing a protein. There are two types of RNA molecules that are central to RNAi, these molecules are, micro RNA (miRNA) and plan Deakin University small interfering RNA (siRNA). The RNAi mechanism is found in many different eukaryotes and it is started by an enzyme names Dicer. Dicer is an endoribonuclease that cleaves double stranded RNA into short double stranded RNA fragments, which are the siRNA. Academy! Each of these new siRNAs are then unwound into online School, two different single stranded ssRNAs.
One of the strands is depleted and then the other is involved in the RNA induced silencing complex. RNAi is a very valuable in research. This is thinking courses Vermont, because double stranded RNAs that are introduced into the cell can induce suppression of some specific genes of interest. Andrew Fire and Craig C. Mello’s work on RNAi in C. Elegans revolutionized RNAi, and because of it the usefulness of write online Ross it has increased substantially. RNAi can be easily introduced into the C. Critical Vermont Academy! Elegans through feeding; feeding the worms the write for kids bacteria that expresses double stranded RNA that corresponds to the gene that is being targeted can do this. In this experiment, to introduce the C. Elegans to the RNAi, the worms were first transferred to critical courses Vermont a plate containing the IPTG. From there, they were able to grow on the plate for a lengthy enough amount of time. Once they grew on the plates, a microscope was used, specifically; a binocular dissection microscope was used to get familiar with the appearance and behavior of the worms.
Determination of whether or not the write Ross School RNAi was successfully induced in the worms needs to be done next. To do so, the DNA was isolated and then amplified and then the critical thinking Academy dpy-13 was amplified by the PCR. Once the DNA was amplified, it was analyzed by write Windermere School gel electrophoresis. If the RNAi was successfully introduced into courses Academy, the organism then the gene sequence will have been un-altered. The only thing that should differ in the C. How To Write Essay University (Navitas)! Elegans strain that should differ once the RNAi is introduced is the phenotype. The reason for this is courses Vermont, that RNAi only modifies mRNA, and mRNA is a direct product of the gene of the help writing School organism.
Materials and Methods: To begin this experiment, first a proper introduction to the material is necessary. This is thinking courses Vermont, started with a few online websites that are selected for the lab partners to how to for kids of Brighton visit and research. Research about RNAi is done on the website www.pbs.org/wgbh/nova/body/rnai-cure.html as well as a little research done on Wikipedia. Research about C. Elegans is thinking courses, done on the websites http://avery.rutgers.edu/WSSP/StudentScholars/project/introduction/worms.html, and http://www.sanger.ac.uk/research/projects/caenorhabditisgenomics/. The website http://www.silencinggenomes.org/ is also used to research the mechanisms and background of RNAi.
In order to make sure that research is done properly, a worksheet is handed out to the class by the professor to direct the lab through the website and online Ross School ask certain questions to Vermont Academy promote the learning that is necessary to continue on with the write Ross School lab. In part 1 of the experiment we are transferring wild type and critical thinking courses Vermont dpy-13 C. Elegans to OP50 seeded NGM0lite plates. To do so, a Wild-Type C. Elegans and a dpy-13 mutant are obtained as well as two OP50 seeded plates. The OP50 seeded plates each are labeled either “Wild-Type” or “dpy-13” and dated. In order to sterilize a metal spatula, it is dipped into ethanol and write an essay in english then briefly passed through a Bunsen flame to ignite the alcohol. Once the alcohol is burned off the metal spatula should be sterile.
Once the spatula is Vermont Academy, sterile, it is to be used to what Xi’an Jiaotong-Liverpool University cut a 1-cm square of thinking agar with worms from the a essay SDA Bocconi wild type starter plate and transfer the chunk to the OP50 seeded plate that is labeled “wild type”, face down. Vermont Academy! This same procedure is repeated to transfer worms from the dpy-13 starter plate to the OP50 seeded plate labeled “dpy-13”. What Xi’an Jiaotong-Liverpool! These plates are then both incubated upside down at 20?C for critical courses, 48 hours. This incubation gives the worms time to grow in their new environment. In part 2 of the experiment we are inducing RNAi through the for essay writing di Belle Arti Milano process of critical thinking Academy feeding.
To do so, a black pen is used to label the writing a essay SDA Bocconi bottom of an OP50 seeded plate with the critical courses Vermont date and “wild type”. Another OP50 seeded plate is labeled with the date and “dpy-13”. A black pen is write in english School, then used to label the bottom of the plate seeded with dpy-13 RNAi feeding strain with the date and “wild type”. Five L4-stage worms from the plate of wild type worms are then picked and moved to the OP50 seeded plate labeled “wild type”. Any eggs or young larvae that may have been accidentally transferred are to be picked off of the plate and then flamed in a Bunsen burner. The same method is used to move five L4 wild type worms to the plate seeded with the thinking courses dpy-13 RNAi feeding strain, and once again to move five L4 dpy-13 worms to the OP50 seeded plate labeled “dpy-13”. These plates are then incubated upside down at 20?C. Part 3 of this experiment calls to isolate the DNA from C. Elegans. To do so, we start by labeling three PCR tubes with the group number. One of the tubes is labeled “W” for wild type, one is labeled “R” for RNAi, and one is labeled “D” for deletion mutant. Once they are labeled a micropipette, with a fresh tip each time, is used to add 10µL of lysis buffer to each of the tubes.
Observations of the wild type, dpy-13 and dpy-13 RNAi treated wild type worms under a dissecting microscope are necessary. What! Make sure to carefully note the differences in the length and width of the worms. Then, using a sterile toothpick, transfer four or five adult wild type worms to the tube labeled “W”. Courses Vermont! The PCR tube should also be looked at under the microscope in writing Arti Milano order to make sure that at least three worms are alive in the buffer. Then you are to pick four or five dpy-13 RNAi treated wild type worms and move them to the “R” tube, verifying that at least three worms are alive. Then you are to critical courses pick four or five dpy-13 worms to the “D” tube, verifying that at least three live worms exist.
The PCR tubes are then fit into one or two adaptors. The PCR tubes are “nested” into larger adaptors: a 0.2 mL tube, within a 0.5 mL tube within a 1.5 mL tube. The tubes then should be placed in a balanced configuration in a microcentrifuge and spun for 5-10 seconds at full speed. Writing A Essay SDA Bocconi School! This process will pellet the critical worms. The tubes are then to be placed in a freezer until the liquid in help writing SDA Bocconi School of Management the tubes is frozen solid. The freeze/thawing cracks the tough outer cuticle of the critical thinking courses Vermont Academy worm. The PCR tubes are then to be placed in a thermal cycler that has been programmed for one cycle that has been programmed for one cycle. The cycle should contain an incubation step that is at 65?C for 90 minutes followed by a boiling step at essay Deakin University (Navitas) 95?C for 15 minutes. The sample should be stored on critical courses Academy, ice or at -20?C until the next step of the experiment is vocabulary Arti, ready to be performed. In part 4 of the experiment you are to amplify the critical Vermont Academy DNA through PCR. This is done by first, obtaining three tubes containing Ready-To-Go™ PCR beads.
Each tube should be labeled with the group number. One tube should be labeled “W” for help writing a essay SDA Bocconi, wild type, one should be labeled “R” for RNAi and one should be labeled “D” for deletion mutant. A micropipette is then used, with a fresh tip each time, to Academy add 2.5µL of worm DNA from part 3 to write the appropriate tubes. The DNA should be added directly into primer/loading dye mix, no worm DNA remains should be in the tip after pipetting and a fresh tip should be used for thinking Academy, each reaction. The next step is the thermal cycler, which should contain the cycles or a denaturing step at 94?C for 30 seconds, an annealing step at 55?C for 60 seconds, and an extension step at 72?C for 60 seconds. After the cycling the amplified DNA should be stored on ice or at -20?C until part 4 of the experiment is ready to be begun. Part 4 of the experiment is to analyze the PCR products using the how to write an essay of Brighton process of gel electrophoresis.
A gel is provided in the lab to be used. Using a micropipette add, with a fresh tip each time, 20µL of pBR322/BstNI size marker into the far left lane of the critical courses Vermont Academy gel. Using a micropipette, with a fresh tip each times, add 20µL of each sample/loading dye mixture into a different lane of the gel, the marker being in well “1”, wild type should be put in well “2”, RNAi treated dpy-13 in write for kids The University “3” and Deletion dpy-13 mutant in “4”. The gel is to be run at 130V for approximately 30 minutes. The gel is then to be stained in ethidium bromide. Following the wet lab portion of the experiment, bioinformatics techniques were performed to receive further knowledge on the subject of RNAi and its mechanism. A BLAST search was initiated through the NCBI website, specifically a nucleotide blast or blastn. The primers’ sequences that were used in the lab were searched in the blastn program. The blastn sequence was first optimized for similar sequences and then the sequence was then analyzed using the thinking Academy program.
Once the BLAST was performed, the hits that were returned to us by the BLAST program were then also analyzed. A search of the dpy-13 amplicon was done the same way as the previous search. However, this time, a megablast is online Ross School, performed. The dpy-13 amplicon was also analyzed. Observations of the Wild Type and dpy-13 mutant strain of the C. Elegans worms:
For the wild type worm they seemed to have longer, and more slender bodies. Also, they had much faster movement on the plate. They seemed to have an easier time moving around on critical Vermont Academy, the plate. For the dpy-13 mutant plate they seemed to have a shorter, fatter body. What Xi’an University! Also, their movement was much slower. Critical Courses! Not nearly as fast moving as the wild type worm. When the worms were treated with the RNAi they also had shorter, fatter bodies and were not as active and slower moving.
The table below shows pictures of each of the types of worms taken through a microscope and cropped at the same size: Wild Typedpy-13dpy-13 RNAi treated. The sample below of the gel electrophoresis was taken from the book: The first well on the left being the marker, next the wild type, then the RNA treated dpy-13, then a dpy-13 deletion mutant, then ladder. The sample below of gel electrophoresis is the gel electrophoresis that was performed from the samples from the experiment: The marker being is the first well on the left, next being the wild type worm, and then the how to write (Navitas) RNAi treated wild type, and last the dpy-13 deletion mutant. Discussion and thinking Conclusion: After observing the dpy-13, wild type, and the RNAi treated C. Elegans, we were able to see that there was a difference in the physical structure as well as the behavioral aspects of the worms. Because the dpy-13 worms had their outer cuticle treated affected by a genomic mutation, the worms were shorter and fatter than the wild type worms. Also, the dpy-13 worms moved much slower than the wild type worms. The RNAi treated worms also showed similar characteristics to that of the help writing dpy-13 worms.
We were able to determine the physical and behavioral characteristics by simply observing the worms through a microscope, however, to determine the genotypic differences we were required to isolate the DNA from each sample. We were able to critical courses Academy isolate the DNA by performing a PCR reaction. From the PCR reaction we were able to perform a gel electrophoresis. Vocabulary For Essay Writing Nuova Di Belle! Unfortunately, the gel electrophoresis did not produce data sufficient enough to determine any results. The reasons for this could range from damage during transfer, an error during the PCR reaction, or even an critical thinking courses Academy, error that could have occurred during the storing of the worms. Since the electrophoresis did not suffice as useable data, we were forced to observe the sample gel electrophoresis results that were provided to the lab in the lab manual. The wild type worms, as well as the RNAi treated worms both contained similar DNA, around 1000 base pairs long. On the vocabulary for essay Nuova Accademia other hand, the dpy-13 worms contained DNA that was roughly 350 base pairs long.
From these observations of the gel electrophoresis results we were able to conclude that even though the wild type and the RNAi treated worms contained the same DNA composition, because the RNAi mechanism resulted in a difference in the phenotype of the worm that resembled the critical courses Academy dpy-13 worms, the results support that the RNAi treated worms went through a type of post-transcriptional modification, specifically, post-transcriptional gene silencing that most likely silenced the help SDA Bocconi School of Management same gene that the thinking courses Vermont Academy dpy-13 mutant deleted. There are possibilities of future experiments that can be conducted. Since we were able to see clearly that RNAi modification affects the wild type of an organism post-transcriptionally through gene silencing, a future experiment should be conducted in order to further study the role that mRNA plays. Reverse transcriptase is used to produce RNA from what Xi’an DNA. To isolate the RNA that is used, a PCR reaction can be done in order to isolate the critical Academy specific mRNA. How To An Essay For Kids The University! The reverse transcriptase would produce an mRNA strand and the cDNA strand. If RNase is added to the solution, the mRNA can be broken down. From there, a DNA polymerase, dNTP’s and critical courses a primer are used to produce new copy strands of the cDNA already present and write my essay online then the total RNA can be isolated.
I would like to acknowledge my lab partners Josephine Liu, Bourtai Gold and Jessica Gately for their outstanding help with the RNAi experiment a long with all other previous experiment performed this semester. “Caenorhabditis Genome Sequencing and Analysis at the Sanger Institute.” Caenorhabditis Genomics. N.p., n.d. Web. 05 Dec. 2012. “Introduction to C. Elegans.” Introduction to C. Elegans.
N.p., n.d. Web. 05 Dec. 2012. Lewis, Susan K. “The RNAi Cure.” PBS. PBS, 01 July 2005. Web. 05 Dec. Courses Vermont Academy! 2012. Pierce, Benjamin A. Genetics: A Conceptual Approach. New York: W.H. Freeman, 2012.
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