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Bacterial Transformation Lab Report Essay. In this lab, the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. How To Writing University Of Hertfordshire. One plate was the control plate, having only the LB or agar for the bacteria and negative pGLO, which is the liquid not containing the plasmid. This is the plate that was compared with the three others in order to identify the critically thinking Wilbraham, changes. The second plate contained negative pGLO, LB, and ampicillin.
This is to see whether or not the bacteria will become resistant to how to start writing examples University (Navitas) the ampicillin and grow, or not be resistant and have no growth. The third plate contained positive pGLO (has the plasmid), LB, and ampicillin. Again, to whether there is resistance to the antibiotic with the difference of a positive pGLO rather than the negative pGLO. The fourth plate contains the positive pGLO, LB, ampicillin, and arabinose (a sugar). One of these plates will have the ability to glow in the dark because the gene making GFP (green fluorescent protein) will be turned on. The whole idea of this gene being turned on comes from the outside of this lab and within the anatomy of the bacteria itself. This bacteria goes through the Edith, process of gene regulation during transcription.
This process turns on only a fraction of the cell’s genes. Writing An Essay (Navitas). When genes are turned off, it is because a repressor binds to critically thinking Wilbraham and Monson an operator in the cell, which stops the process of transcription. This occurs when high levels of tryptophan bind to the repressor causing it to an essay University of Hertfordshire bind again to the operator. When a gene is Edith Cowan University turned on, a metabolite binds to the repressor, which stops it from binding to the operator and allows transcription to continue, turning the gene on. In one of these plates, the gene will be either turned on or off based on what is added to the bacteria. Materials and Methods. See science manual Bacterial Transformation Lab for complete list of materials and procedures. There are colonies because the pGLO contains the plasmid, which allows the bacteria to survive and become resistant to the ampicillin. Has a beige color.
Also contains pGLO with plasmid, so the bacteria is resistant to the ampicillin. Arabinose is also present. The bacteria on this plate glow in the dark almost a greenish color. No bacteria at how to writing of Hertfordshire (Navitas), all. The plasmid was not present, therefore the bacteria was not resistant to the antibiotic. TNTC, plate is full of persuasive Arts University Bournemouth beige bacteria. Start Writing Examples University Of Hertfordshire (Navitas). Based on the results, there are conclusions that can be made about what is seen. In Writing Edith Cowan. The plate with +pGLO LB/Amp was resistant to the antibiotic. How To Start An Essay Examples Of Hertfordshire. There were colonies all over the plate due to the resistance.
This comes from the plasmid in harvard CATS Canterbury the pGLO, which allows the bacteria to transform and become resistant to the ampicillin. How To Start Writing An Essay Examples. The plate with +pGLO LB/Amp/Ara is the critically thinking Wilbraham and Monson, plate with the bacteria that glows. As explained in the introduction, this is because the gene to make GFP was turned on. The arabinose put into the bacteria helped to start writing University (Navitas) make the GFP. Without the arabinose, the bacteria would not be able to make the GFP. This plate also expressed resistance to CATS College the ampicillin like the first plate. The third plate contained –pGLO LB/Amp. This plate showed no bacteria at all. This is because there was no plasmid to make the bacteria become resistant. In the fourth plate, the bacteria count was TNTC because it only contained –pGLO and LB, there was no change made to the bacteria. Analyzes these changes came easy because there was a control plate (-pGLO LB) to compare all the transformations to.
For this lab, there is also many questions and math involved. One question is, which organism is better suited for how to start examples University (Navitas), total genetic transformation- single celled or multi-celled? The best answer is a single-celled organism because that one cell would be able to take up a new gene. Another question is, which would be the best choice for critically thinking Wilbraham, a genetic transformation: a bacterium, earthworm, fish or mouse? The best answer for this question would be a bacterium, since they are single-celled and they reproduce rapidly. Examples Of Hertfordshire. There is also a lot of a conclusion for an math involved. Starting with the LB/Amp/Ara plate, the amount of cells is 53 found on the plate. To then find the total amount of pGLO DNA (ug), the start writing an essay, concentration of DNA (ug/ul) needs to be multiplied by the volume of DNA (ul). In this case .08 x 510, summing up to 40.8 ug.
The next step is to find the fraction of DNA used by dividing volume spread on history of Exeter, LB/Amp plate (ul) by the total volume in the test tube (ul). This would be 100 / 510, summing up to 10/51 or .2. How To Examples (Navitas). After, the pGLO DNA spread (ug) must be found by multiplying the total amount of DNA used in “ug” by the fraction of DNA used. So, 40.8 x 10/51, this sums to 8.16 ug. This leads to our ultimate goal of the on writing St. Johnsbury, transformation efficiency by dividing the total number of cells growing on the agar plate by the amount of DNA spread on writing an essay examples (Navitas), the agar plate. Journal. This would be 53 / 8.16, summing to 6.5 as our transformation efficiency. Overall, the lab was very successful.
All the results came out as they were supposed to. The +pGLO with LB/Amp/Ara has glowing bacteria due to the transformation it went through. The arabinose helped turn on a gene with transcription in start writing University (Navitas) order to make GFP (green fluorescent protein), which was expressed when the UV light was shown onto help University the bacteria giving it a green glow rather than all the other bacteria. There was a resistance to ampicillin in this plate. In the plate containing +pGLO LB/Amp, there was also a resistance to the ampicillin. This is how to writing an essay University (Navitas) because a plasmid was present to transform the bacteria into being resistant to the antibiotic present. In the plate containing –pGLO LB/Amp, there was no bacteria found on in writing University, the plate.
This is because a plasmid was not present to transform the bacteria to start writing an essay University become resistant. In Writing An Essay Edith University. On the plate containing –pGLO LB, there was TNTC with bacteria. This is because it is the control plate and how to start an essay University (Navitas) nothing in the bacteria or the plate was changed or transformed. From this lab, there were no errors. Everything ran perfectly and smoothly.
If this lab was done over again, it would most likely be done on a larger scale. Example, maybe more bacteria growth or more transformations so the changes become really clear. Also, it could be used to see what other genes can be turned on or off. Uc A-g Courses Durham. All in all, the lab was fun and a success in how to start examples University of Hertfordshire transforming the tips on writing for an essay St. Johnsbury, bacteria, e-coli, into one that is antibiotic resistant and into one that can glow. 1)Bacterial Transformation Lab. Parafilm. American National Can Co. 2015. 2)Operons. An Essay Of Hertfordshire. AP Biology Homework Handouts.
Notes. 2015. On Writing A Conclusion For An Essay Academy. 3)#8221;LabBench.#8221; LabBench. Pearson Inc, n.d. Web. 03 Mar. 2015. University/College: University of Chicago. Type of paper: Thesis/Dissertation Chapter. Date: 15 September 2016. Let us write you a custom essay sample on Bacterial Transformation Lab Report.
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cover letter for nursing. Should i include collegiate sports in my resume? And if so where? It depends how much professional experience you have. If you are a recent college grad, then it is acceptable to include on your resume. A Conclusion Essay St. Johnsbury? Good luck on the job hunt! Good luck on how to start examples (Navitas) the job hunt!
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Childhood Obesity — The Shape of how to start writing an essay University of Hertfordshire Things to Come. David S. Ludwig, M.D., Ph.D. N Engl J Med 2007; 357:2325-2327 December 6, 2007 DOI: 10.1056/NEJMp0706538. Interview with David Ludwig on the impact of the childhood obesity epidemic. (6:01) Last week, I met with the G. family in the Optimal Weight for Life (OWL) clinic at my hospital. One of the parents was overweight, and on writing St. Johnsbury Academy, the other was obese. The five children were more severely obese and had numerous weight-related complications — one had evidence of fatty liver, one had high blood pressure, two had gastroesophageal reflux, two had orthopedic problems, three had marked insulin resistance, four had dyslipidemia, and all had emotional problems related to their weight. Sadly, this family might be a microcosm of 21st-century America: if we don't take steps to reverse course, the children of each successive generation seem destined to be fatter and sicker than their parents. How will obesity affect the physical and psychological well-being of children in how to start writing an essay examples of Hertfordshire (Navitas) coming decades?
What effects will childhood obesity have on life expectancy, the national economy, and our society? To explore these questions, one might view the a conclusion, obesity epidemic as consisting of four overlapping phases. Phase 1 began in how to an essay examples (Navitas) the early 1970s and steps an essay Edith, is ongoing: average weight is progressively increasing among children from all socioeconomic levels, racial and start writing examples University (Navitas), ethnic groups, and regions of the country. Today, about one in a level University of Exeter (INTO) three children and adolescents is overweight (with a body-mass index, or BMI, in the 85th to 95th percentile for age and sex) or obese (BMI above the 95th percentile), and the proportion approaches one in two in certain minority groups. 1 Though it has attracted much attention from the how to start writing examples University, medical profession and the public, childhood obesity during this phase has actually had little effect on public health, because an obese child may remain relatively healthy for years.
Phase 2, which we are now entering, is essay characterized by the emergence of serious weight-related problems. How To Writing Examples University! 2 The incidence of type 2 diabetes among adolescents, though still not high, has increased by a factor of more than 10 in the past two decades and may now exceed that of type 1 diabetes among black and Hispanic adolescents. Fatty liver associated with excessive weight, unrecognized in the pediatric literature before 1980, today occurs in about one in CATS College three obese children. Other obesity-related complications affecting virtually every organ — ranging from crippling orthopedic problems to sleep apnea — are being diagnosed with increasing frequency in children (see table Complications of Childhood Obesity. ). There is also a heavy psychosocial toll: obese children tend to examples of Hertfordshire, be socially isolated and have high rates of disordered eating, anxiety, and steps University, depression. When they reach adulthood, they are less likely than their thinner counterparts to complete college and are more likely to live in poverty. It may take many years to reach phase 3 of the epidemic, in which the medical complications of obesity lead to life-threatening disease. As Baker et al. (pages 2329–2337) and Bibbins-Domingo et al. (pages 2371–2379) report in this issue of the writing an essay of Hertfordshire (Navitas), Journal, overweight or obesity in childhood or adolescence increases the risk of coronary heart disease (CHD) in adulthood; by 2035, Bibbins-Domingo et al. predict, the prevalence of CHD will have increased by 5 to 16%, with more than 100,000 excess cases attributable to increased obesity among today's adolescents. Tips A Conclusion St. Johnsbury Academy! Preliminary data from Canada suggest that adolescents with type 2 diabetes will be at high risk for limb amputation, kidney failure requiring dialysis, and premature death.
In some, fatty liver will progress to hepatitis and cirrhosis, which may remain asymptomatic until irreversible organ damage has occurred. Poverty and social isolation would complicate the an essay of Hertfordshire, timely identification and management of and Monson Academy such problems. Shockingly, the risk of dying by middle age is already two to three times as high among obese adolescent girls as it is how to start writing of Hertfordshire (Navitas) among those of persuasive essay help Arts (AUB) normal weight, even after other lifestyle factors are taken into how to start examples University of Hertfordshire (Navitas), account. History A Level University! 3 My colleagues and I have predicted that pediatric obesity may shorten life expectancy in the United States by 2 to how to start examples University of Hertfordshire (Navitas), 5 years by midcentury — an effect equal to that of all cancers combined. 4. Without effective intervention, phase 4 of the thinking Wilbraham and Monson Academy, epidemic will entail an acceleration of the obesity rate through transgenerational mechanisms. Obese children tend to how to start writing of Hertfordshire, be heavy in tips on writing a conclusion for an essay Academy adulthood, in part because obesity-promoting habits persist.
In addition, carrying excessive weight early in life may elicit irreversible biologic changes in hormonal pathways, fat cells, and the brain that increase hunger and adversely affect metabolism. Furthermore, adult obesity and an essay examples University, its complications appear to increase the risk of obesity and its complications in tips a conclusion for an essay Academy offspring through nongenetic influences, a phenomenon termed perinatal programming. For example, a recent study found that maternal hyperglycemia during pregnancy strongly predicted BMI in offspring at 5 to 7 years of age, after adjustment for maternal weight gain and birth weight. 5. Currently, the economic costs of pediatric obesity in the United States are relatively small — probably several hundred million dollars annually. Writing An Essay Examples Of Hertfordshire! Without effective intervention, the costs of obesity might well become catastrophic, arising not only from escalating medical expenses but also from diminished worker productivity, caused by physical and journal College Canterbury, psychological disabilities. Future economic losses could mean the difference between solvency and how to writing University (Navitas), bankruptcy for tips essay Academy, Medicare, between expanding and shrinking health care coverage, and between investment in and neglect of our social infrastructure, with profound implications for our international competitiveness. The human costs would be incalculable. Like global warming, the obesity epidemic is a looming crisis that requires action before all the scientific evidence is in. And as with climate change, some have questioned experts' forecasts, doubting the examples of Hertfordshire (Navitas), far-reaching impact of obesity, though skepticism is gradually being overcome by accumulating data. Others would defer concerted efforts to address the problem, placing hope in the development of new drugs or surgical procedures that, like some abundant and nonpolluting energy source, might offer a painless technological fix.
Or they argue that the costs of action are too great, not recognizing that our survival depends on essay help Arts Bournemouth, solving the problem. But I believe that obesity differs in one important respect from global warming: simple solutions are available, and with a comprehensive national strategy, we may be able to implement them without great sacrifice. Certainly, we have much to learn about the regulation of body weight. Low-fat diets have yielded disappointing results, and very-low-carbohydrate diets appear to be more effective only in the short term. Novel approaches that focus on the quality rather than the ratio of macronutrients appear promising, and other areas warrant study, including the effects of sleep deprivation, stress, infectious agents, and endocrine-disrupting environmental toxins on weight. Unfortunately, the how to start writing an essay University of Hertfordshire (Navitas), U.S. government has thus far invested only a fraction of a cent in research for every dollar that obesity costs society.
And although broad consensus exists regarding the dietary and lifestyle habits needed to prevent and treat childhood obesity, we lack anything resembling a comprehensive strategy for encouraging children to eat a healthful diet and engage in physical activity. Such a strategy would include legislation that regulates junk-food advertising, provides adequate funding for decent lunches and steps an essay Edith University, regular physical activities at school, restructures the farm-subsidies program to favor nutrient-dense rather than calorie-dense produce, and mandates insurance coverage for preventing and treating pediatric obesity. Parents must take responsibility for their children's welfare by how to writing examples University of Hertfordshire (Navitas), providing high-quality food, limiting television viewing, and modeling a healthful lifestyle. But why should Mr. and Ms. G.'s efforts to history a level coursework of Exeter (INTO), protect their children from life-threatening illness be undermined by massive marketing campaigns from the manufacturers of junk food? Why are their children subjected to the temptation of such food in the school cafeteria and vending machines? Why don't they have the opportunity to exercise their bodies during the school day? And why must Mr. and writing, Ms. G. fight with their insurance company for reimbursement to cover the costs of their children's care at the OWL clinic? Fortunately, with the exercise of help Arts University Bournemouth (AUB) both personal and social responsibility, we have the power to choose the shape of things to come.
An interview with Dr. Ludwig can be heard at www.nejm.org. Dr. How To Writing Examples University Of Hertfordshire (Navitas)! Ludwig is director of the Optimal Weight for Life Program in the Division of Endocrinology, Children's Hospital Boston, and an associate professor of pediatrics at Harvard Medical School — both in steps Boston. Ogden CL , Carroll MD , Curtin LR , McDowell MA , Tabak CJ , Flegal KM . Prevalence of overweight and obesity in the United States, 1999-2004. JAMA 2006;295:1549-1555.
Ebbeling CB , Pawlak DB , Ludwig DS . Childhood obesity: public health crisis, common sense cure. Lancet 2002;360:473-482. van Dam RM , Willett WC , Manson JE , Hu FB . The relationship between overweight in adolescence and premature death in women. Ann Intern Med 2006;145:91-97. Olshansky SJ , Passaro DJ , Hershow RC , et al.
A potential decline in life expectancy in the United States in the 21st century. N Engl J Med 2005;352:1138-1145. Hillier TA , Pedula KL , Schmidt MM , Mullen JA , Charles MA , Pettitt DJ . Childhood obesity and metabolic imprinting: the ongoing effects of how to an essay examples (Navitas) maternal hyperglycemia. Diabetes Care 2007;30:2287-2292. Collynn F. Woeller, E’Lissa Flores, Stephen J. In Writing An Essay Cowan! Pollock, Richard P. Start Writing An Essay Examples University Of Hertfordshire! Phipps. . (2017) Editor’s Highlight: Thy1 (CD90) Expression is Reduced by the Environmental Chemical Tetrabromobisphenol-A to Promote Adipogenesis Through Induction of microRNA-103. Toxicological Sciences 157 :2, 305-319.
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