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Essay: Gene transfer techniques – pros and cons. Gene transfer is a personal Concord, a technique to stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by which DNA is transferred from a donor to a recipient. Once donor DNA is inside the write faster, recipient, crossing over write essay Concord can occur. The result is a recombinant cell that has a genome different from either the donor or the recipient. A recombination event must occur after transfer in order that the change in the genome be heritable (passed on to the next generation).The genes are the blueprints essential to generate all the write essays faster, proteins in our bodies which eventually perform all the biological functions. Therefore, when a gene is efficiently introduced into a target cell of the host, the protein which is encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and a personal essay Concord its function.

A range of techniques and naturally occurring processes are utilized for the gene transfer. Techniques are available for the introduction of DNA into many different cell types in culture, either to study gene function and regulation or to research review article The University, produce large amounts of recombinant protein. Cell cultures have therefore been used on a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and viral coat proteins for immunization.The most important application of College this technology is in vivo gene therapy, i.e. the introduction of DNA into writing Ashville College the cells of live animals in order to treat disease. The extraordinary potential of altered DNA molecule is to give rise to new life-forms that are better adopted for survival. Change in base sequence of DNA leads to change in protein causing disease condition which can be corrected by manipulation of gene. Much emphasis has been given to the gene manipulation in write a personal essay, cardiovascular diseases, parkinson’s disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. The most straightforward is direct DNA transfer, the physical introduction of foreign DNA directly into the cell.

For example, in cultured cells this can be done by microinjection, whereas for cells in vivo direct transfer is prime essay Ashville College, often achieved by bombardment with tiny DNA-coated metal particles. The second route is write essay College, termed transfection, and this encompasses a number of how to write School of Business & Management techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from their surroundings. The third is to write essay Concord College, package the research article, DNA inside an. animal virus, since viruses have evolved mechanisms to naturally infect cells and introduce their own nucleic acid. The transfer of write Concord foreign DNA into a cell by Ashville College, this route is termed transduction. The choice of methods of a personal College DNA transfer depends upon the target cells in which transformation will be performed.

It also depends upon the objectives of how to faster School gene manipulation. The transfection may be either stable or transient. Although, choice of write essay College DNA transfer method is how to write IUBH School, very important, the other important steps are selection of gene, isolation of a personal gene, preparing recombinant DNA and selection of transformed cells. The regeneration of essay writing Ashville College organism with new characteristics is also equally important. The delivery of genes in vitro can be done by treating the write a personal College, cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of these methods. These are the powerful tools for research and have possible applications in gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of how to a contract law essay Newcastle new technologies has made gene delivery both more efficient yet more confusing given the formidable array of a personal College reagents and methods now available. Determining the best gene delivery approach for any particular experiment requires careful consideration of a number of different factors as stated below. 1. Cellular Context or Environment – The context or environment of the cells (i.e., in vivo vs. in.

vitro vs. ex vivo) to which nucleic acids will be delivered is the how to write Newcastle University, first factor to write a personal essay College, consider when. deciding upon a gene transfer method. The obstacles and challenges posed by delivery to how to faster Lucton, in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and techniques for overcoming them. For example, targeting nucleic acids to. particular cells while avoiding other cells is usually a major concern for in vivo gene transfer.

applications, sometimes a concern for ex vivo applications, but usually not a concern for in vitro. Another important issue that must be addressed when planning in vivo (but not in vitro) gene. delivery studies is the essay College, immunogenicity of the gene transfer system. Write Faster Lucton School. This is particularly important when considering the College, use of certain viral vectors discussed previously, which may be highly immunogenic. Also related to immunogenicity is the issue of general safety, both for the experimenter and for the subject of any study utilizing transfection. Almost always, the use of viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type – The origin of the write essays faster School, cells or tissue to essay Concord College, which nucleic acids will be.

transferred is typically the second parameter considered when selecting a gene delivery. method. For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is always important to review the literature to. determine if the cell type of interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of cells actually transfected or transduced, with each method.

Certain cell types are often described as ‘notoriously difficult to transfect’. These include primary cell cultures of essay many varieties. Successful gene transfer to such cells. usually requires a good deal of research before selecting any particular gene delivery method. 3. Delivery Efficiency – For most applications, transfecting or transducing as many cells as.

possible is preferred, especially when it is necessary to detect the write a personal essay Concord, presence of rare transgene. products. Thus, it is important to prime writing College, determine the write a personal, level of delivery efficiency required for a given. application before deciding on a particular gene transfer method. In most cases, it is found that. viral vectors provide the highest delivery efficiencies. For some commonly used and easily transfected cell lines, cationic lipids nearly match viral vectors in writing a history Leysin American School, their gene transfer efficiency, while providing safer and much quicker procedures.

4. Essay Concord College. Stable vs. Write Essays Faster School. Transient Gene Delivery – Gene transfer procedures are frequently categorized. by whether the delivered gene remains separate from the host cell chromosome or whether it is integrated into the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of a personal essay Concord time – usually within. several days – because the expression vector is either degraded or expelled from the host cell. In the how to, second case, known as stable gene delivery, expression of the a personal essay, transferred gene is.

prolonged, or stable, because the vector is integrated into the host cell chromosome. How To Write. Obviously, different applications require different time periods of transgene expression. Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression.

In the case of viral vectors, determining whether a particular virus is suited for stable or transient. transduction is simply a matter of learning the general characteristics of the vector. In the case. of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature.

5. Type of Delivered Molecule – Another factor to a personal essay College, consider when selecting a gene delivery. method is what type of nucleic acid or other molecule is how to write titles in essays Moreton Hall School, being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides. It is frequently easiest to simply review the literature to learn what methods have been used successfully, and to contact other researchers who have experience delivering the molecule of interest. 6. Cytotoxicity – Another issue that comes up frequently when choosing among different gene. delivery methods is the cytotoxicity of the various methods and reagents. Some methods, such.

as electroporation and gene guns, can be extremely harsh on cells and often result in the death. of a majority of cells. Write A Personal Essay College. Also, the DEAE-dextran can be quite harsh on some cell types, especially. when a DMSO or glycerol shock step is used as part of the procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.

not a problem. In the majority of applications however, a high percentage of cell death is simply. not acceptable. The plethora of available cationic lipids and polymer reagents have varying. degrees of toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to minimize cell death. 7. Suspension vs. Adherent cells – Most commonly transfected cells grow – and Ashville are. transfected – while attached to write College, some type of support, whether that support is the tissue from. which they are derived, some inert membrane or scaffolding, or the surface of a tissue culture. plate.

Less common are cells that grow while suspended in prime Ashville, medium, such as those derived from. blood and write a personal other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to a contract law essay Newcastle, rely on harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when. possible due to their simplicity and cost-effectiveness. 8. Expertise – The degree of difficulty in performing the various transfection techniques can. vary significantly, and therefore the expertise and write a personal experience of the researcher is usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and polymer reagents. Also, of the chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in experimental conditions.

9. Time – Another important factor to consider is the time required for gene delivery. How To Titles In Essays Moreton Hall School. Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from essay, two weeks to one month to complete. In contrast, chemical and physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Thus, it is not uncommon to how to write law essay University, have to weigh the benefits of faster results and write a personal essay less labor against the benefit of higher efficiency. 10 Cost – Finally, cost can be a major factor when deciding on the best gene delivery.

method for a given application. Essay. The most expensive methods tend to be the. physical/mechanical methods, such as electroporation and gene guns, due to the specialized. equipment they require.Viral gene delivery is perhaps the second most expensive method, since it requires the vector reagents, extensive time and write a personal Concord labor to complete lengthy vector preparation procedures, as well as proper disposal of viral waste materials. The third most expensive gene delivery methods utilize polymers and how to University cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in the hundreds of write essay College dollars, depending on the number of how to faster Lucton transfections to be completed.

Finally, the least expensive methods utilize calcium phosphate, which is a cheap and abundant mineral, and naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the a personal essay Concord, naturally occurring process of gene transfer which involves absorption of the Newcastle, genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the native DNA resulting in College, the genetic expression of the received DNA. Transformation involves the uptake of “naked” DNA (DNA not incorporated into structures such as chromosomes) by how to Newcastle University, competent bacterial cells. Cells are only competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to the completion of cell wall synthesis . Transformation is usually a natural method of gene transfer but as a result of write Concord technological advancement originated the artificial or induced transformation. Thus there are two types called as natural transformation and artificial or induced transformation. In natural transformation, the University, foreign DNA attaches itself to the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. The presence of nucleases restricts the entry of two strands of the a personal essay Concord, DNA, destroys a single strand thus allowing only one strand to enter the host cell. Any DNA that is prime Ashville College, not integrated into the chromosome will be degraded. This single stranded DNA mingles with the a personal, host genetic material successfully.

The artificial or induced method of transformation is done under laboratory condition which is either a chemical mediated gene transfer or done by electroporation.. Genetic engineers are able to induce competency by putting cells in certain solutions, typically containing calcium salts. Research Review. At the entry site, endonucleases cut the a personal essay Concord College, DNA into fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into single strands. The single-stranded DNA may recombine with the host’s chromosome once inside the cell. This recombination replaces the prime Ashville College, gene in the host with a variant – albeit homologous – gene. DNA from a closely related genus may be acquired but, in a personal essay College, general, DNA is not exchanged between distantly related microbes. Not all bacteria can become competent. Conjugation is a means of gene transfer in writing Ashville, many species of bacteria.

Cell-to-cell contact by a specialized appendage, known as the F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to a cell that does not contain the plasmid. On rare occasions an write a personal Concord College F-plasmid may become integrated in the chromosome of writing essay Leysin School its bacterial host, generating what is known as an a personal essay Hfr (high frequency of recombination) cell. Such a cell can also direct the law essay Newcastle, synthesis of a sex pilus. As the chromosome of the Hfr cell replicates it may begin to cross the pilus so that plasmid and write chromosomal DNA transfers to a history Leysin School, the recipient cell. Such DNA may recombine with that of its new host, introducing new gene variants. Plasmids encoding genes for write essay Concord, virulence factors antibiotic-resistance are passed throughout populations of bacteria, and between multiple species of bacteria by conjugation. In the write titles of books in essays Moreton Hall, laboratory, conjugation can be used to transfer disrupted genes on a self-transmissible plasmid, to write Concord College, develop a mutant strain. A gene of interest from a recipient E. coli strain is cloned into a self-transmissible vector and maintained in a donor strain for genetic manipulation. The deleted gene construct is then transferred by prime essay writing Ashville College, conjugation from the donor strain back into write a personal essay Concord the recipient strain. Transduction is another method for research article, transferring genes from one bacterium to another; the transfer is mediated by Concord College, bacteriophages. Ashville College. A bacteriophage infection starts when the virus injects its DNA into a bacterial cell.

The bacteriophage DNA may then direct the synthesis of write essay Concord College new viral components assembled in the bacterium. Bacteriophage DNA is replicated and writing a history essay School then packaged within the phage particles. Early in the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Some of these fragments of bacterial DNA are packaged within the bacteriophage particles, taking the place of a personal essay College phage DNA. As the number of phages increases it breaks open (lyse) the write faster School, cell. When released from the infected cell, a phage that contains bacterial genes can continue to infect a new bacterial cell, transferring the bacterial genes. Sometimes genes transferred in this manner become integrated into write a personal essay the genome of their new bacterial host by homologous recombination. Such transduced bacteria are not lysed because they do not contain adequate phage DNA for viral synthesis and these phages can enter an alternate life cycle called lysogeny. In this cycle, all the virus’s DNA becomes integrated into the genome of the host bacterium. The integrated phage, called a prophage, can confer new properties to the bacterium. Researchers use transduction as a means of gene transfer as it requires a media like virus for transferring genes from one cell to the other.

Viruses are thus as a tool to introduce foreign DNA from the selected species to the target organism. There are two types of transduction called as generalized transduction in which any of the bacterial gene is transferred via the bacteriophage to the other bacteria and specialized transduction involves transfer of limited or selected set of genes. Viral vectors for gene transfer: The use of viruses as vectors for transduction, i.e. the how to of books in essays Moreton School, introduction of genes into animal cells by exploiting the natural ability of the virus particle, within which the a personal essay Concord, transgene is packaged, to adsorb to the surface of the cell and gain entry is being employed . Due to how to write a contract Newcastle University, the efficiency with which viruses can deliver their nucleic acid into cells and the high levels of replication. and gene expression it is possible to a personal Concord College, achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for gene transfer to write essays faster Lucton, living animals.

Four classes of viral vector have been developed for use in human gene therapy and have reached phase 1 clinical trials. Concord College. These are the retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Before introducing the individual vector systems,we discuss some general properties of viral transduction vectors. Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by replacing one or more viral genes. This is research review article, generally achieved either by ligation (many viruses have been modified to incorporate unique restriction sites) or homologous recombination. For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as ‘gutless vectors’) contain just the cis-acting elements required for packaging and genome replication. The advantage of such vectors is their high capacity for write Concord College, foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. The choice of vector depends on the particular properties of the virus and article the intended host, whether transient or stable expression is required and how much DNA needs to be packaged. Write A Personal Essay Concord. For example, icosahedral viruses such as adenoviruses and titles of books in essays Moreton Hall retroviruses package their genomes into preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. A Personal College. Conversely, rod-shaped viruses such as the baculoviruses form the capsid around the genome, so there are no such size constraints.

There is of books in essays Moreton Hall School, no ideal virus for gene transfer ‘ each has its own advantages and disadvantages. In recent years, a number of hybrid viral vectors have been developed incorporating the beneficial features of two or more viruses. 1) viral vectors that especially efficient at transducing the write Concord College, intended cell type but not other cell types. 2) retroviruses are particularly proficient at how to write a transfer essay IUBH of Business & Management delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic. TRANSFECTION BY CHEMICAL METHODS. One of the methods of gene transfer where the write a personal, genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and the gene is transfection.

This mode of gene transfer involves creation of pores on how to faster School, the cell membrane enabling the cell to receive the a personal essay Concord College, foreign genetic material. When transformation is carried out in eukaryotic cells it is termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. . When the research review article of Brighton, two are combined, a fine precipitate of the positively charged calcium and the negatively charged phosphate will form, binding the write a personal, DNA to be transfected on its surface .Cells are then incubated with precipitated DNA either in solution or in tissue culture dish. A fraction of a transfer essay of Business & Management cells will take up the calcium phosphate DNA precipitate by Concord, a process not entirely understood but thought to be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of 1-2 % . It can. be increased if very high purity DNA is used and the precipitate allowed to write essays faster Lucton School, form slowly. 1. Frequency is write a personal essay College, very low.

2. Faster Lucton. Integrated genes undergo substantial modification. 3. Many cells do not like having the solid precipitate adhering to them. and the surface of their culture vessel. 4. Integration with host cell chromosome is random. 5, Due to above limitations transfection applied to write essay Concord, somatic gene therapy.

This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of identifying many oncogenes. 2)DNA transfer by DAE-Dextran method: DNA can be transferred with the help of DAE Dextran also. DAE-Dextran may be used in how to write essays faster Lucton School, the. transfection medium in which DNA is present. This is a polycationic, high molecular weight substance and write a personal essay College is convenient for transient assays. How To Write In Essays Hall School. The negatively charged DNA binds to the polycation and essay College the complex is taken up by the cell via endocytosis.

If DEAE (diethylaminoethyl )-Dextran treatment is coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the transferred gene. The advantage of this method is that, it is cheap, simple and can be used for transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the The University, technique are its relative simplicity and speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of cell growth and induction of heterogeneous morphological changes in cells. Furthermore, the concentration of write a personal essay Concord serum in the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for the production of stable transfectants. For cells that grow in suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of writing Leysin School DNA by polycation-DMSO: As calcium phosphate method of DNA transfer is reproducible and a personal efficient but there is narrow range of optimum conditions.

DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to review, increase the adsorption of write essay DNA to the cell surface followed by a brief treatment with 25-30% DMSO to increase membrane permeability and enhance uptake of DNA. In this method no carrier DNA is required and stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to transport molecules into the cells. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes.

They are considered as spheres of lipid bilayers surrounding the molecule to be transported and promote transport after fusing with the cell membrane. Liposomes for use as gene transfer vehicles are prepared by adding an a transfer appropriate mix of bilayer constituents to an aqueous solution of DNA molecules. In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. The liposomes are then ready to be added to target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the most common and essay generally utilized gene transfer technique in the recent years and it utilizes cationic lipids. The combined DNA and cationic lipids act instantaneously to review article The University of Brighton, form structures called as lipoplexes that are more complex in write a personal Concord College, structure than the simple liposomes. Write In Essays Moreton School. Cationic lipids have a positive charge and Concord these form cationic liposomes which interact with the titles of books Hall, negatively charged cell membrane more readily than uncharged liposomes. Essay. This leads to a fusion between cationic liposome and the cell surface resulting in quick entry by endocytosis and the delivery of the DNA directly across the plasma membrane.

Cationic liposomes can be produced from how to write a transfer School & Management, a number of cationic lipids that are commercially available and sold as an in vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of a personal Concord College lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the neutral helper lipids, which are generally included along with the how to essay IUBH School of Business & Management, cationic lipid. This allows entrapped DNA to escape the endosomes, reach the nucleus and get access to the cell’s transcriptional machinery.The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids’ buffering action within the endosomes and by the fusion of the write a personal, lipid with the endosomal membrane. The ability of a lipid to destroy endosomes is one of the main characteristics of a transfection reagent. 2. Long term stability.

4. Protection of nucleic acid from degradation. 5. particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 6. cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to terminally differentiated cells. A rapid and simple technique for introducing genes into a wide variety of microbial, plant and animal cells, including E. coli, is electroporation. Electroporation uses electrical pulse to produce transient pores in the plasma membrane thereby allowing macromolecules into the cells. These pores are known as electropores which allow the molecules, ions and water to pass from one side of the membrane to another. The electropores reseal spontaneously and the cell can recover. The pores can be recovered only if a suitable electric pulse is applied .The formation of how to University electropores depends upon the cells that are used and the amplitude and duration of the electric pulse that is applied to them. When subjected to electric shock, cells take up exogenous DNA from the suspending solution. Once inside the write a personal essay Concord, cell, the DNA is integrated and the foreign gene will express.

A proportion of these cells become stably transformed and can be selected if a suitable marker gene is carried on how to, the transforming DNA. The most critical parameters are the intensity and duration of the electric pulse, and these must be determined empirically for different cell types. Electric currents can lead to dramatic heating of the cells that can results in cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and highly reproducible. Many different factors affect the efficiency of electroporation, including temperature, various electric-field parameters (voltage, resistance and capacitance), topological form of the DNA, and various host-cell factors (genetic background, growth conditions) and post-pulse treatment. General applications of electroporation. 1. Write Essay Concord College. Introduction of exogeneous DNA into animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast. 2. Electroporation can be used to increase efficiency of transformation or transfection of bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method.

4. How To Faster. Genes encoding selectable marker may be used to introduce genes using electroporation. 5. Write A Personal Essay College. To study the transient expression of molecular constructs. 6. Electroporation of early embryo may result in essays faster Lucton School, the production of Concord transgenic animals. 7. A History Essay American. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and B lymphocytes can be transformed by this technique. 8. Naked DNA may be used for gene therapy by applying electroporation device on animal cells. Advantages of electroporation. 1. Method is write a personal College, fast. 3. Applied for a number of cell types. 4. Simultaneously a large number of write IUBH cell can be treated. 5. High percentage of stable transformants can be produced.

1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to place the electrodes deep into the internal organs. 3)High voltage applied to write essay Concord College, tissues can result in irreversible tissue damage as a result of how to Moreton thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is required that can accurately control pulse length and amplitude.

4) Larger numbers of cells may be required than for write a personal essay, other methods because, in many cases, the most efficient electroporation occurs when there is up to 50% cell death. Sonication is the act of applying sound energy to agitate particles in a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the use of these ultrasonic frequencies for how to of books Hall, modifying the write a personal College, permeability of the cell plasma membrane. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Research The University Of Brighton. Although the early results could achieve only 10- to 30-fold increases in transfection efficiency in vitro, technical refinements have lead to enhancements of up to several thousand fold in essay College, vitro ,sufficient to encourage ultrasonication mediated gene transfer in a transfer essay IUBH School & Management, vivo. 1) It is non-invasive and well tolerated, 2)extraordinary safety record over write a personal a wide range of frequency and intensity. 3)high levels of public acceptability and understanding.

there are highly sophisticated, flexible, cost-effective and readily available diagnostic and writing essay American therapeutic systems that can achieve site-specific transfer of ultrasound energy almost anywhere in the body, except perhaps the a personal essay College, lung. 4) The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. 5) in vivo is relatively nontoxic. 1)Extended exposure to low-frequency (MHz) ultrasound has been demonstrated to write Newcastle University, result in complete cellular death (rupturing) as well as microvascular hemorrhage and disruption of tissue structure, thus cellular viability must also be accounted for when employing this technique. 2) Whether ultrasound affects later steps in the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is write a personal essay College, under active study for the introduction of School foreign genes in tissue culture cells, especially mammalian cells.

Sonoporation is also being studied for use in targetedGene therapy in vivo, in a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. In microinjection DNA can be introduced into cells or protoplast with the help of a personal essay College very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the DNA injected may be taken up by prime essay writing College, the nucleus. The desired gene in the form of plasmid or alone is injected directly into the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Write A Personal Essay Concord College. Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. How To Write Law Essay. Computerized control of write essay holding pipette, needle, microscope stage and video technology has improved the efficiency of this technique.

Advantages of microinjection. 1. Frequency of stable integration of how to titles of books in essays DNA is far better as compare to other methods. 2. Method is effective in transforming primary cells as well as cells in established cultures. 3. The DNA injected in essay Concord, this process is subjected to less extensive modifications. 4. Mere precise integration of recombinant gene in limited copy number can be obtained.

2. Skilled personal required. 3.Has not been successful for many plant cells . 4. A History Leysin American School. Embryonic cells preferred for manipulation. 5. Write A Personal College. Knowledge of mating timing, oocyte recovery is essential. 6. Method is useful for protoplasts and not for the walled cells. 7. Process causes random integration. 8. Rearrangement or deletion of host DNA adjacent to review The University of Brighton, site of integration are common.

9. The technique is essay Concord, laborious, technically difficult, and a history Leysin limited to the number of cells actually injected. Applications of microinjection. 1. Process is applicable for plant cell as well as animal cell but more common for animal cells. 2. Technique is ideally useful for essay Concord, producing transgenic animal quickly. 3. How To A Contract Law Essay Newcastle University. Procedure is important for gene transfer to embryonic cells. 4. Applied to inject DNA into plant nuclei. 5. Method has been successfully used with cells and protoplast of. tobacco, alfalfa etc. Fig: microinjection in write a personal Concord College, mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to.

deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is relatively new and essay writing Ashville novel method amongst the a personal essay College, physical methods for artificial transfer of exogenous DNA. The nucleic acid is delivered through membrane penetration at a high velocity, usually connected to microprojectiles like microscopic particles of write essays faster tungsten or gold coated into write College cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the research review, particles pass through the cell, the DNA becomes free to integrate into the plant-cell genome. The gene gun is a device that literally fires DNA into target cells . The DNA to be transformed. into the cells is coated onto microscopic beads made of either gold or tungsten. Beads are carefully. coated with DNA.

The coated beads are then attached to a personal Concord College, the end of the plastic bullet and loaded into. the firing chamber of the gene gun. An explosive force fires the bullet down the barrel of the gun. towards the target cells that lie just beyond the end of the barrel. When the bullet reaches the end of. the barrel it is caught and stopped, but the DNA coated beads continue on toward the how to write law essay Newcastle University, target cells. Some of the beads pass through the cell wall into the cytoplasm of the target cells.

Here the bead and. the DNA dissociate and the cells become transformed. A Personal Essay Concord. Once inside the target cells, the DNA is. solubilised and may be expressed. 1. Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by biolistic method. 3. Mitochondria of prime writing plants and chloroplast of chlamydomonas have been. 4. Method can be applied to filamentous fungi and yeast (mitochondria).

5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. This approach has been shown to be effective for transferring transgenes into mammalian cells in write a personal, vivo . 1. Requirement of protoplast can be avoided. Unlike electroporation and microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue. 2. Walled intact cells can be penetrated. 3. Manipulation of genome of subcellular organelles can be achieved. 4. This is also a highly mechanized or robotic mediated technique in which the. speed of the micro-projectile particles is controlled by foolproof mechanisms.

5) It is also gaining in use as a method for a history Leysin, transferring DNA construct into whole animals. 6) This technique is fast, simple and safe, and it can transfer genes to a wide variety of tissues. 7)there appears to be no limits to the size or number of write genes that can be delivered. 1. Integration is random. 2. How To Write Essays Faster Lucton. Requirement of equipments. 3.It can be a challenge to obtain a sufficient number of write essay Concord College cells modified by this method to essay writing Ashville, see a biologically significant effect. A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.

At the site of the beam impact, due probably to local temperature changes, the cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by changing the irradiation time and/or the radiation intensity (energy), appear on the wall, last for a short time and fade spontaneously . It takes a fraction of a second for the aperture to essay Concord College, close, long enough to writing essay American School, allow the foreign DNA, contained in write Concord, the medium, to slip into the cell. It is research review The University, well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Plasmid DNA (circular DNA residing in bacterial cytoplasm) is used as a vector of the gene of interest. We inject the plasmid into target tissue, on which a laser target is placed, and College subject the target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and hence an LISW. By placing optically transparent material on the target, the plasma is confined, resulting in how to write of books Hall School, an increase in the LISW’s impulse.

Interaction of tissue cells with the LISW allows plasmid to enter the cytoplasm. 1) it only takes advantage of the presence of phenol-red, a normal cell medium component, with no need of addition of extraneous substances; (2) it is a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of a personal Concord College selected cells even in the presence of titles of books Moreton Hall cells of different type (providing that they are morphologically distinguishable). 4) . it is rapidly replacing virus mediated techniques as they have serious side effects caused by immune response and limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the a personal Concord, efficiency of cell modification by orders of essay writing magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the Concord College, genes can be introduced into article of Brighton the cells. With the advent of molecular tools and technologies it is now comparatively easy to a personal College, introduce gene into cells without losing its integrity and biological activity. Moreover the recent development in molecular biology has made the transfer of gene with great accuracy to the target cell. The transfer of gene through different gene transfer technologies has cured a number of diseases. Research is on progress to cure those diseases which cannot be cured by using drugs.

Moreover the treatment of diseases by gene transfer provides better result for a prolong period of time. It is the write law essay, need of hour to discover new and cheap method of gene transfer technologies so to make the treatment of the diseases a little easier and affordable. Improvements in gene transfer are required in terms of transfection approaches to allow improved transgene uptake efficiencies. 1)Society of Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN.

Medical Biotechnology Division, School of Biosciences and write a personal Concord College Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of prime essay writing Ashville gene manipulation: R.W.Old ,S.B. Primrose and R.M.Twymann ‘ sixth edition,chapter 10 and 11. 3) BIOTECHNOLOGY 2020-From the Transparent Cell to. the Custom-Designed Process–Prof. Write. Gerhard Kreysa Dr.-Ing. E.h. Dr.h.c.

Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING. 5)From genes to genomes- concepts and applications of DNA technology–Jeremy W Dale, Malcom von Schantz. Search our thousands of essay writing Ashville essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question? There are UK writers just like me on a personal, hand, waiting to help you. Each of write Lucton us is qualified to a high level in our area of expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and you could have your customised Science work in essay College, your email box, in as little as 3 hours. This Science essay was submitted to us by a student in order to help you with your studies. This page has approximately words.

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Tip Sheet: An Admissions Dean Offers Advice on write Writing a College Essay. Periodically, in a feature called “Tip Sheet,” The Choice will post short items by write admissions officers, guidance counselors and others to help applicants and their families better understand aspects of the admissions process. As an inaugural post in this series, Martha C. Merrill, the dean of a personal essay Concord College, admission and financial aid of Connecticut College, and a graduate of the class of 1984, encourages incoming high school seniors to begin contemplating their college essays this summer. She also offers perspective on what she looks for in an applicant’s essay. Prospective students will often ask me if a good essay will really get them accepted. Article Of Brighton? The truth is a personal Concord, that while no essay will make an unqualified student acceptable, a good essay can help a qualified applicant stand out write a transfer School of Business from the a personal essay Concord College, competition.

A good essay just might be what turns a “maybe” into a “yes.” The college application process takes time, preparation and creativity, which is a lot for any active senior to handle. Summer, however, typically offers about 10 weeks free of classes and essay Leysin American, homework and many of the other stresses that come with high school. The pressure of the looming college application deadline is still months away, which allows students the freedom to a personal essay College, play around with different ideas, test different angles and solicit feedback from friends and family. Another reason to focus your summer energy on how to a contract law essay Newcastle University crafting a quality essay: at this point in the admission process, it is write essay Concord College, one of the few things you can still control. This is your chance to show us what you are capable of when you have time to think, prepare, rewrite and polish. While there is no magic formula for the perfect admission essay, there are a few things prospective college students should know. Here are my Top Ten tips: Write about yourself . A great history paper on the Civil War might be very well written, but it doesn’t tell me anything about the writer. Write A Transfer Essay IUBH School? Regardless of the topic, make sure you shine through your essay.

Use your own voice . I can tell the difference between the voice of write essay, a 40-year-old and a high school senior. Focus on one aspect of yourself . If you try to cover too many topics in Ashville College, your essay, you’ll end up with a resume of activities and attributes that doesn’t tell me as much about you as an in-depth look at write College, one project or passion. Be genuine . Don’t try to impress me, because I’ve heard it all. Just tell me what is important to you. Consider a mundane topic . Sometimes it’s the simple things in life that make the best essays. Some of my favorites have included essays that reflect on the daily subway ride to school, or what the family goldfish observed from the fishbowl perched on the family kitchen table. It doesn’t have to be a life-changing event to be interesting and a contract law essay, informative.

Don’t rely on essay College “how to” books . Use them to get your creative juices flowing, but don’t adhere too rigidly to their formulas, and definitely don’t use their example topics. While there are always exceptions, the essays faster Lucton, “what my room says about me” essay is write Concord, way overdone. How To A Transfer Essay IUBH Of Business? Share your opinions, but avoid anything too risky or controversial . Write A Personal Essay? Your essay will be read by a diverse group of individuals from a wide range of backgrounds, so try to appeal to how to a transfer School & Management, the broadest audience possible. Tell a good story . Show me why you are compassionate; don’t tell me you are. Show me that you have overcome great difficulty; don’t start your essay with “I have overcome great difficulties.” Don’t repeat what is already in your application . If you go to a performing arts school and all of your extracurricular activities and awards relate to a personal College, dance, don’t write about how much you love dancing. Tell me something I couldn’t know just from reading the other parts of your application. Finally, don’t forget about the supplements . The supplement questions are very important – you should plan to spend as much time on them as you do on your essay.

A well-written essay won’t help if your supplement answers are sloppy and uninformative. If you’ve been through this process before — either as a practitioner, student or parent — and would like to how to a contract law essay, add, or respond, to Ms. Merrill’s list, use the comment box below. Essay Concord College? If you’d like to propose a future subject for “Tip Sheet” — one you’d want to read, or perhaps even propose writing — please send a short email message to us at how to essay School of Business & Management, thechoice@nytimes.com. Comments are no longer being accepted. Another piece of advice is to write a personal essay Concord, have someone proofread your essay, but DO NOT have multiple people give you advice about the essay. It is easy to spot the review article, student essays that have been “finished” or “edited” by friends and family.

And the write essay, worst essays are the write faster, ones that have been edited to meet the comments of multiple readers. As a faculty member who regularly catches students plagiarizing on Concord class assignments, the application essay is writing essay Leysin, a hint at that student’s future approach to writing assignments. I think you could do your readers a big favor by compiling a list of states that offer an early path to write College, college. Here in Texas, it’s called the Texas Academy of Mathematics and Science (TAMS). It’s a two-year program at article of Brighton, the Univ. of North Texas in Denton for write a personal essay Concord College high school juniors #038; seniors; it has about 400 students total. Basically, the students take their first two years of college, and get credit for their last two years of high school. My wife and I have two children, both graduated from TAMS. Best thing is, the prime essay writing Ashville, state covers tuition, fees, and books; only write a personal College, cost to us was room #038; board and a program fee. check it out by write of books School googling “tams”. I have some PDFs I could email you, too, if you’re interested. Dripping Springs, TX.

All nicely said but if all this was true there would have been the huge numbers of write, essay coaches or books we have out today. Ask the entrants to any Ivy league school about whether they used coaches or guides and you will get a different answer. it would be good to start thinking about this college application essay… (still a year away) Ms. Merrill’s Top Ten tips are an excellent guideline for the college admissions essay. I’m currently a college sophmore and faster Lucton School, vividly recall going through this process. One additional tip I would add is keep it lite. I think college admissions panels are tired of reading about a personal essay Concord College how you spent your summer wielding a hammer for Habitat for Humanity or ladling soup in a homeless shelter. Forget the write essay, essay – play a sport and be really, really good at it. A Personal Essay? If you are also a decent student that will be your ticket. Trust me — athletes have a huge competitive advantage in the college admissions game.

The admissions people won’t come right out and how to a contract University, say it, they’ll say things like “We try for balance in a personal essay, every class”, but if Amherst doesn’t beat Williams, Yale doesn’t beat Harvard, etc. Law Essay? etc., they will get a lot of pressure. If the violin section is a little off one night, or the lead in the play is a bit weak, it won’t really matter. I’m not cynical, just realistic. I would avoid grammatical errors such as Martha’s “I can tell the difference between the voice of a 40-year-old and a high school senior” and Mary’s (reader 1) double error: “As a faculty member who regularly catches students plagiarizing on class assignments, the application essay is a hint at write a personal, that student’s future approach to a contract law essay, writing assignments.” I’m certain that Martha meant “I can tell the essay Concord, difference between the voice of a 40-year-old and that of a high school senior,” and that Mary meant “As a faculty member who regularly catches students plagiarizing on class assignments, I note that the application essay is a hint at students’ future approaches to writing assignments.” the best thing you can do is try out a lot of ideas. my english teacher senior year made us write a different personal essay every day for the first month of how to write of books Hall School, school. i never would have thought of my ultimately successful topic if i hadnt been for being forced to do so much writing. if you really feel you must start over essay the summer, try out lots of research review article, ideas and dont commit. ask an english teacher or recent ivy grad for advice, your parents may not have the best sense of a personal, a relevant and write University, not trite topic.

once you have your topic, draft and Concord College, fine tune. Essay Leysin American? i went through at least 40 full drafts. luckily my topic answered every essay question i came across. finally, i think the mundane topics advice is write a personal Concord, risky. Review Article The University Of Brighton? true, you can have a very good essay on a mundane topic, but you also run the risk of write essay, sounding like everyone else and being trite. if you are writing about how to write your subway ride as a metaphor for your dreams in life, it had better be a really sparkling, innovative essay. These tips, while a good guide, are confusing. If some of the best essays she’s read include what your life is like from the POV of a goldfish on write a personal College the kitchen table, then what’s wrong with a creative essay on how to write a transfer essay what my room says about a personal Concord me? That story can be equally as creative. As a 60-year-old graduate student who has also been a journalist for 11 years and recently published a book about write essay IUBH School & Management my life, I believe in the power of personal story. Not all teens have found their “voices” yet, and so they try on write essay others for prime writing Ashville College size, so of course that will come through the essay. But your voice is unique. It’s you. A Personal? Don’t try to be anybody else. So… Keep it simple, be honest, use more verbs than nouns and review The University of Brighton, avoid adjectives and adverbs as much as possible.

Tell how an experience you had made you feel and what you learned from it. Describe what sets your heart on fire. As the parent of two college-aged sons, I could not agree with this advice more. One wrote about a challenge that he overcame and the other about being compassionate. Both essays were about events that happened in their everyday school lives. Both were written in active voice and write a personal essay College, were little windows into their characters.

Neither used the how to School, words challenge or compassionate. I am convinced that it was the write a personal essay College, strength and sincerity of their essays that opened the doors at the top schools that said “Yes” to my sons. Writing A History American School? The essays were the differentiating factor in all the numbers that are part of an application. I encourage other parents to suggest that their kids just be themselves in their essays – small is good, generalities are boring, tell about something that makes you you. Write Essay? Oh, and read The Gatekeepers – – it offers the best insights into the college admissions process of any of the dozens of books I read on the topic. I wonder if, at the most competitive colleges/universities, anything makes a difference beyond sociology: My daughter is a National Merit Finalist (actually won a National Merit Scholarship at a school she chose not to attend), had an “unweighted” GPA over 3.9 at how to Newcastle University, a magnet International Baccalaureate program, had an SAT score of 2290 with an 800 in critical reading (only took it once), had three “5” scores on AP exams before her senior year and every SAT II over 700.

She was accepted at every small college she applied. Rejected at write a personal Concord College, Harvard, Yale, wait-listed at Columbia (and then told there is no place) and at Duke….but then, 25 of 26 applicants from her (public) school were rejected from prime essay writing Ashville, Yale…the one acceptance: a superb musician, triple-legacy, Presidential Scholar. She IS an athlete, but not good enough to play at the schools that rejected her (and plans to at the small college she will attend). Oh…she’s upper middle class white (and competing in a major metropolitan area against many white legacy kids at these top schools). “been through it” might think himself realistic for advising that everyone get ‘really really good’ at a sport, but being a non-sports person with a non-sports kid, our (IMO rather more sure-fire) resolution to this particular problem is – – apply only to technical colleges; they do not require essays. Tongue only partly in cheek… Not all Ivy League admits use coaches or guides- I didn’t. I took a risk in writing my application three years ago- I wrote a genuinely personal essay. It was frightening for me to do bec it revealed things about write a personal Concord my background that I wasn’t sure Harvard could handle. But it was a risk that paid off. So, my perspective is- take a risk, expose yourself, share why admission truly matters to write Lucton School, you.

Thank you, Ms. Concord? Merrill. As a parent whose daughter is at the very very beginning of this process, I’d love to a transfer essay IUBH School of Business, see more advice on the admissions process from you — please keep it coming! And thank you NYT for passing along practical, applicable information. Most college admission officers agree that a student’s character is the most difficult thing to measure on write a personal essay Concord College the application. College essays are the place for students to writing essay American, reveal their personal stories in an authentic, engaging and sincere way . In addition to what has already been mentioned, it’s important to read the a personal College, essay prompts carefully and understand the intent of the question. Jeannie Borin, M.Ed. Some advice that not every student would need, but could be helpful to many: 1 – Don’t try to sound too “intellectual,” if that means stuffing the essay with high-brow vocabulary that you would never use in an ordinary conversation.

If you sound like you’re trying to impress the reader with this vocabulary, you probably are – negatively. 2 – If you were sweating and how to a contract Newcastle University, stewing with your essay, try another draft version in a “devil may care” frame of mind. That is, just write it quickly with whatever comes into your head (on the topic) without caring if the essay is good and bad. Then let a trusted person compare the versions. Sometimes the latter turns out to have the better “flow,” and you can improve on that in a personal essay, the editing process. It’s a little cynical to a history American School, suggest that all Ivy League admits use coaches and essay Concord College, guides.

A friend currently at Yale was told by a college counselor that her essay was terrible (it read too much like a “story” and didn’t have a “message”), and she sent it anyway. Article The University? I wrote my essay on my own and got into a school famous for its English program. Probably the best advice is to a personal essay Concord College, stay far away from writing College, “moral of the write Concord College, story” lines. I’ve seen plenty of books that praise essays about the writers’ overcoming of obstacles, blah blah blah, with all their lines about “through my experience, I learned…” I would get pretty darn bored of that if I were an admissions officer. Neither of my children used a coach or had special classes. How To Write Essays Lucton School? We checked the write Concord College, grammar and spelling on how to write essays faster their essays and write a personal Concord College, let them focus on writing Ashville their interests in and out of school. One is at Concord, an Ivy one is at a small, tier one school. Writing Essay Leysin? I expect the Concord College, one going to the small school will get the prime essay writing Ashville College, better education. Some advice that not every student would need, but could be helpful to a personal College, many:

1 – Don’t try to prime writing College, sound too “intellectual,” if that means stuffing the essay with high-brow vocabulary that you would never use in an ordinary conversation. If you sound like you’re trying to impress the reader with this vocabulary, you probably are – negatively. 2 – If you were sweating and stewing with your essay, try another draft version in write, a “devil may care” frame of mind. That is, just write it quickly with whatever comes into your head (on the topic) without caring if the how to Hall School, essay is good and bad. Then let a trusted person compare the versions. Sometimes the write a personal College, latter turns out to have the better “flow,” and you can improve on that in the editing process.

P.S. How To Of Business & Management? – Sorry, forgot to tell you great post! Barbara’s #2 is a great solution to any time one gets ‘writer’s block’. Essay Concord College? From Thank You notes to funding proposals, it works. Also, the write a contract Newcastle University, ‘trusted person’ who reads and essay, edits the outcome of the “devil may care” effort can be oneself – but not until the next day. Read the essay to someone else. Read it as if you are telling a story.

You will hear what sounds clumsy, and you will see if it captures attention. I suggest that a good deal of how to write of books Moreton, time and money would be saved if admissions officers would simply put all those applicants who seem to meet standards for a college education into a lottery. That random choice is fair and write a personal essay Concord, is better than officers playing God as to who is fit to adorn their insitution, My College Admissions Essays: 1. What work of art, music, science, mathematics or literature has surprised, unsettled or challenged you? : A 64-slice CT scanner that the a transfer essay School of Business & Management, local hospital that I volunteered at had just gotten. 2. Write A Personal Concord College? What’s your favorite word and why?: Determination (Probably not the “best” choice but it really is and I think I showed it was genuine)

3. Choose any topic of your choice: I wrote a descriptive essay of my trip to write a transfer essay IUBH of Business & Management, the Eiffel Tower and how I was blown away by the beauty and a personal essay, grandeur of the structure. And this was also an essay that I had used in how to a contract law essay, an English class for a writing contest and my teacher had rated the paper as an write a personal essay College, A+ so hey, it was probably my best writing. Currently at the University of Virginia as a 3rd year student. Admissions does sometimes seem like a lottery… And Doc? Does your daughter’s school mascot happen to be a rocket? I think I go to the same school, based on the information you gave. As someone who was admitted to some Ivy Leagues and waitlisted at some Ivy Leagues, and who played a sport but was not recruited, I must say that the essay was probably the prime essay Ashville, key in helping me stand out from the thousands of middle-class, white, suburban applicants. The essay is a personal essay, really one of the only aspects of the how to write a contract law essay, application in Concord, which you can show who you really are. Resumes are nice, but they show what you do–which is meaningless unless this provides insight into who you are and how your character’s been shaped by what you do. I’m sure many people would probably make blanket statements that are hard to understand…”don’t write about something too grandiose” “don’t write about something too mundane” “don’t make it too intellectual-sounding” “don’t make it sound like intellectualism is not a part of your life”–but the best advice I can give is write titles Hall School, figure out write essay College a writing style that works for you, and run with it.

If you look hard enough, you will find people in your life who know you well enough to give you tips on your writing style while staying true to yourself and making it genuine. Take this advice with a grain of salt. Consider it carefully and remember…colleges are not looking to accept your neighbor, or your English teacher, or your friend’s mom who works at a newspaper. They are looking for true insight into your character, and you should seize this opportunity to reveal what it is that makes you who you are.

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A Master Essayist Releases a New Collection. Write A Personal College? A Literary Education and Other Essays . By Joseph Epstein. Axios, 2014. 537p. A History Essay American School? HB, $24. During my years as a newspaper books editor, I periodically made regional literary pilgrimages close to my Mobile, Alabama, home. These visits became feature stories about places rich in literary history, such as Savannah, Georgia, Oxford, Mississippi, and write Concord, New Orleans. A Contract Law Essay University? But much like poet Henry David Thoreau, who remarked how he had “travelled a good deal in Concord,” I also explored a few little-known local sites.

One of essay, my mini-jaunts was a night spent in the Thomas Byrne Memorial Library on Mobile’s Spring Hill College campus. Founded by the Jesuits in 1830, Spring Hill College was the first Catholic college in the Southeast. Its library (alas, since moved) was housed in a marvelous 1930 edifice that was a scaled-down version of Thomas Jefferson’s Virginia statehouse. Grand Palladian windows surrounded the cavernous reading room, and the books, many dating from the nineteenth century, were snugly housed in a six-level shelf system at one end. These stacks were a delicious haven for any book lover. Little yellow bulbs illuminated the aisles, and the air was suffused with the smell of old paper and leather. A narrow staircase enclosed in a history Leysin American School, wire mesh ran through the center, with an electric dumbwaiter alongside. To be locked in alone with tens of thousands of books and no obligation other than to write essay Concord, follow my own whims was an research of Brighton unforgettable experience.

During the course of the evening, I plucked beautifully bound first editions by write a personal essay John Ruskin, Henry Adams, Henry James, and research The University of Brighton, Edith Wharton and lovingly admired their gorgeously illustrated, foxed pages. Well into the wee hours, I stumbled downstairs and a personal College, stretched out on an overstuffed leather couch, with several copies of the American Scholar to a contract Newcastle, speed me into dreamland. And it was there that I discovered Joseph Epstein, or Aristides, as was his pen name in those days in that publication. I have since reflected on what a perfect environment that was for an introduction to Epstein, perhaps one of our best contemporary essayists. Essay Concord College? Leaving aside that it was a Catholic institution and Epstein is writing, Jewish, everything else resonated—the building’s distinguished architectural pedigree, the reverential respect with which older books were retained and made accessible, and the august reading room with everything arranged around the sacred act of reading. To read Epstein is to essay Concord College, know that such things matter—tradition, books, culture. To say that I have been a fan ever since would be an understatement. It is therefore with great pleasure that I note Epstein’s latest book, A Literary Education and Other Essays . Included are thirty-eight pieces reprinted from a variety of publications such as Commentary , the New Criterion , the Weekly Standard , and the Wall Street Journal . As one would guess from such sources, Epstein is culturally conservative, but happily not a scold. His prose is fluid, funny, and always worth attending. Write In Essays Hall School? The earliest selection dates from 1969, but most of the rest are from the early twenty-first century.

They are divided into thematic chapters on College, memoir, culture, the arts, education, language, magazines, and intellectuals. Within these categories, the selections span an interesting array of subjects—old age, stand-up comics, contemporary poetry, intellectual freedom, the New Yorker , the liberal arts, Walter Cronkite. Write A Transfer? Like most essay collections, this one is best appreciated in measured doses, a few pieces at a sitting. Marathon readers will find that the selections generally hang together well, though there is some repetition of anecdote and phrase. Of especial interest are Epstein’s eyebrow-raising revelations about his personal past. Though recently castigated for a Wall Street Journal piece in which he mourned the demise of the “WASPocracy” in a personal, American life (not included in this collection), Epstein led a youth that was about as far from that world as can be imagined. He grew up comfortably enough in a “predominantly Jewish, wholly middle-class atmosphere.” His father was a salesman, and both parents practiced an The University of Brighton attitude of bemused detachment toward his daily affairs. Write Essay Concord? When he once told his mother, “a highly intelligent woman of even temperament,” that he was bored, her response was, “Really? May I suggest that you knock your head against the wall. Write Essays Faster Lucton? It’ll take your mind off your boredom.” He was a bit of a hellion as an adolescent, riding around Chicago with his pals, visiting brothels. His first prostitute was Leona, “truly striking, biracial,” and a heroin addict. “The lush air of corruption surrounding women like Leona was always at least as enticing as the write College sex,” he reveals, “which in fact tended to a contract University, be quick and, to essay Concord, put it mildly, perfunctory.” In one of the earliest essays in the book, “Memoirs of a transfer of Business & Management, a Fraternity Man” (1971), originally published in Commentary , Epstein revisits his collegiate self at the University of Illinois.

His attire then was classic preppie: “a brown Harris tweed jacket, a maroon cashmere sweater, a careful rep tie over write essay a white button-down oxford cloth shirt, Cambridge gray trousers with a small buckle in the back, and how to write essay IUBH School & Management, plain-toed cordovan shoes.” His socks were black “and rode high up on the calf.” If this is what readers familiar with his later career trajectory would expect, Epstein insists in a personal College, another essay that his education at Illinois and later at the University of Chicago was “slapdash, wildly uneven, and chiefly autodidactical.” But, he assures the reader, this wasn’t necessarily a bad thing. The most a university can really be expected to do, he says, “is point its students in the right direction: let them know what the intellectual possibilities are and give them a taste of the best that has been thought and written in the past.” Many of these pieces turn on the academy and prime College, the life of the mind. Epstein’s impatience with such things as student evaluations (“useless intrusions into university teaching by the political tumult of the 1960s”), political correctness, and declining standards will no doubt have old-school types cheering. Unfortunately, some of these selections are too narrowly focused to hold much interest for those outside the ivory tower. One such essay in particular, “A Case of Academic Freedom” (1986), is a cri de coeur against write a personal Concord College, a specific instance of political correctness at Northwestern University, where Epstein taught English for decades, but it is how to write Newcastle, lengthy, arcane, and dated to boot.

Happily, it’s the exception rather than the rule here. On the page, Epstein aspires to be one of the “laughing skeptics” and mostly succeeds. A Personal? He is mistrustful of “large ideas, and especially idea systems,” but his touch is light, and he dishes out the bons mots without meanness or acerbity. One may not always agree with his judgments, but they are rendered with art and write titles of books, wit. For example, his definition of a “middlebrow” is write Concord, “anyone who takes either Woody Allen or Spike Lee seriously as an artist.” Contemporary poetry is “slightly political, heavily preening, and not distinguished enough in language or subtlety of thought to be memorable.” He likes Henry James (though his enthusiasm for The Princess Casamassima strikes me, a student of writing, James myself, as somewhat unusual), Willa Cather, and write essay Concord, Ralph Ellison. Held in low regard, “second- or third-rate writers,” are Kurt Vonnegut, Toni Morrison, Jack Kerouac, and write faster Lucton School, Adrienne Rich. In his essay “What to Do about the write Arts?” (1995), originally published in Commentary , Epstein relates the story of write essays faster Lucton, how, after Bill Clinton’s first inauguration, he was contacted by a British journalist for an opinion about the now-late poet and memoirist Maya Angelou. Write Essay College? The man was surprised when Epstein said that he had no opinion about Angelou because he didn’t read her and a history essay American, knew no one who did.

When the reporter asked how that could be, Epstein explained that “there were a number of authors who were not actually for reading but only for teaching, of whom Angelou, who herself teaches, lectures for vast fees, and probably has more honorary degrees than James Joyce had outstanding debts, is decidedly one.” By Epstein’s lights, Angelou’s stature was the result of too many modern university courses that favored contemporary writers working in themes of “race, class, and gender” over the DWEMs (dead white European males). As a result, he argues, cultural judgment is a personal essay Concord, now “skewed,” and “fewer and fewer people are able to how to write essay IUBH of Business, make the important distinctions which high art itself requires for its proper appreciation.” This is Epstein in full arbiter elegantiae mode. For those who might bristle, it should be noted that Epstein at write a personal essay College least stimulates debate, thus fulfilling his charge as an review article of Brighton engaged contemporary essayist. Even when dismissive, as in write essay Concord College, the foregoing instance, Epstein avoids the writing a history essay Leysin American School pepper shaker. He doesn’t have a big mouth and essay Concord College, isn’t overly impressed with himself: “A world filled with people like me would be intolerable.” He’s forceful, yes, but humorous and just self-deprecatory enough to remain grounded and winning. At seventy-seven, he grumbles that everything sure seemed better in the old days—the seriousness of politicians, music, poetry, painting, academic standards—but is essay Leysin School, quick to write a personal, quote George Santayana’s remark that one of the reasons the old complain about the world is because they cannot “imagine a world being any good at all in essay Leysin American School, which they will not be around to participate.” You’ve got to admire a man who can admit that. Welcome to the world of Joseph Epstein. Write A Personal Essay Concord? John S. Sledge is an architectural historian with the Mobile Historic Development Commission in Alabama. He served as books editor for the Mobile Press Register for seventeen years.

His most recent book is Southern Bound: A Gulf Coast Journalist on write titles Moreton School, Books, Writers, and Literary Pilgrimages of the Heart (University of South Carolina Press). Vonnegut second or third rate? Perhaps Vonnegut's populist leanings served to write a personal essay, distort Epsteins judgment. After all, anyone who would engage in coitus with heroin addicted prostitutes has established that his judgment is, shall we say, a bit suspect. Mr. A History American? Epstein was right about Maya Angelou. Not to a personal essay Concord College, disparage her work, but the uncritical wave of praise for essays faster any poet based on race alone indeed skews the write essay Concord true value of the work.

Is Ms. Angelou in the pantheon of contemporary poets? Only because her time had come and politicians such as Clinton adminstered the laurel wreath to pry more votes from write University those who never managed to read her works but believed she had a place in the forefront because of her color. I would think being a token to be used by politicians would have disgusted an intelligent person like herself. A Personal College? I know Epstein from a contract law essay Newcastle University his Norton Book of Personal Essays , required reading in classes for students of creative non-fiction. He and Harold Bloom ( The Western Canon , etc.) represent the write a personal College best and the worst in modern literary criticism, adherents of the old-school, disdainful of the increasing inclusion of the formerly excluded, meaning writers who come from a myriad of cultural and ethnic backgrounds. John S. Sledge handles Epstein's taint of white-male privilege quite well. In the research article day, being told to knock your head against the wall was a typical response from a parent to a personal essay Concord College, a male teen in a Jewish home.

I have no opinion about Epstein because I haven't read him and School, I know no one who has. Did any of his sons turn out to write Concord, be gay? Like all denials, the rejection of 'big ideas' or 'big idea systems' makes a positive statement in reverse that is how to in essays Moreton Hall School, just what it tries to deny, that human beings as critics and rejecters make big ideas and generate big idea systems. The intellectual drive to full interoperability moves toward establishing an essay College 'organon', as we used to say in of books Moreton Hall, the discipline of philosophy. And the effort, while always doomed in absolute historic terms, is nevertheless a generous attempt of write essay Concord, a good soul to try to how to titles of books in essays Moreton School, explain things in our world to College, ourselves and others. Generosity is quite often a good thing. We can all posture as 'laughing skeptics'. How To Titles Moreton School? both laughter and write essay Concord College, skepticism are also good things generally speaking. But smugness and posturing tend not to be. They are as absolute as self-expression as. Epstein is how to Newcastle University, OK in write a personal essay Concord, small doses, but he is very predictable. the whole contemporary art is garbage thing is tiresome. every generation of critics thinks the new art is bad.

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